LAUSR

The optimum hydrolysis conditions of 2.5% (w/v) soy protein isolate (SPI) with 1% (w/w of SPI) Flavourzyme(superscript ®) for increasing glycerol release in mature 3T3-L1 adipocytes were investigated by response surtface methodology (RSM). Higher glycerol release indicated higher lipotysis-stimulating activity. The independent variables were hydrolysis lime (HT) 19.2-220.8 min, pH 5.32-8.68 and reaction temperature (RT) 33.2-66.8℃. Based on the response surface and contour plots, the optimum hydrolysis of SPI with Flavourzyme(superscript ®) for maximizing the glycerol release in the cells occurred at pH=7.12. RT=48.77℃ and HT=124.85 min. The F-value for tack of fit was not significant (p＞0.05), so the second order model was appropriate for describing the response surface. In addition, the model had a satisfactory coefficient of R^2 (=0.935) and was verified experimentally.