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An HPLC-FLD method to measure ochratoxin A in teff (Eragrostis tef) and wheat (Triticum spp.) destined for the local Ethiopian market

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Wheat (Triticum spp.) is among the top cereal grains in terms of production and consumption in Ethiopia. It can be typically infected with ochratoxin A (OTA) producing fungi both in… Click to show full abstract

Wheat (Triticum spp.) is among the top cereal grains in terms of production and consumption in Ethiopia. It can be typically infected with ochratoxin A (OTA) producing fungi both in the field and during storage resulting in animal and human health problems. While there is a wealth of information on the presence of OTA in wheat, the incidence of OTA in teff (Eragrostis tef), a cereal grain produced and consumed traditionally in Ethiopia and Eretria, remains insufficient. The purpose of this study was to develop and validate a high performance liquid chromatography (HPLC) method for OTA analysis in teff and wheat flours and to analyse the characteristic distribution of OTA in teff and wheat flours samples destined for local consumption in Ethiopia using a survey-approach. Wheat and teff flour samples were examined for OTA contamination. OTA was detected in 20% of the teff flour samples and in 50% of the wheat flour samples with median contamination levels of 1.04 µg/kg (limit of detection (LOD) = 0.78 µg/kg...

Keywords: triticum spp; eragrostis tef; wheat triticum; wheat; teff eragrostis; teff

Journal Title: World Mycotoxin Journal
Year Published: 2017

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