Flow cytometry is a versatile technique for analyzing stroke-induced changes in the immune system. Unlike other methods of cell identification such as immunohistochemistry, the technique is rapid, highly sensitive, and… Click to show full abstract
Flow cytometry is a versatile technique for analyzing stroke-induced changes in the immune system. Unlike other methods of cell identification such as immunohistochemistry, the technique is rapid, highly sensitive, and capable of quantifying multiple markers in cell suspensions. Utilizing cell sorters, flow cytometers can also produce highly enriched populations of viable cells for functional studies. The aim of this perspective is to appraise current flow cytometry methods in the field of stroke, provide guidance on best practices, and outline some of the future applications of the technique in pre-clinical and clinical stroke research.
               
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