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Aims and Objectives: The objective of this study was to assess the oral health attitudes and behavior among health professionals (dentists, physicians, nurses, technicians, and pharmacists) working in Prince Sultan Military Medical City (PSMMC), Riyadh, using Hiroshima University Dental Behavioral Inventory (HU-DBI). Materials and Methods: This cross-sectional descriptive study evaluated the oral health attitudes and behavior of health professionals working in PSMMC in Riyadh, Saudi Arabia. A convenient sample of 500 health professionals, such as dentists, physicians, nurses, technicians, and pharmacists working in the PSMMC, Riyadh, was considered for the study. This study used the HU-DBI created by Kawamura to measure oral health attitudes and behaviors among health professionals working in PSMMC. All the data analysis for this study were carried out using IBM-SPSS, version 21.0. Results: A total of 500 questionnaires were distributed among health professionals in PSMMC, Riyadh, and all of the questionnaires were filled and returned back. Thus, a response rate of 100% was obtained. Among all the health professionals considered in the study, highest mean HU-DBI score was observed among pharmacists (6.09 ± 0.85) and dentists (6.08 ± 0.62), followed by physicians (6.06 ± 0.96), technicians (5.90 ± 0.93), and nurses (5.39 ± 1.33). Conclusion: Based on the findings from this study, in general, health professionals considered from PSMMC showed fair oral health attitudes and behaviors as measured by using HU-DBI.