LAUSR

SNP analysis of PPARD -87T/C. A total number of 822 tissue specimens were carefully taken and used to amplify the 239 bp fragment of PPARD by PCR with specific primers (Figure 2A). In RFLP analysis, the -87 T>C polymorphism of PPARD can be determined via BslI digestion. Specifically, PPARD -87 T>C with T/T type cannot be digested by BslI restriction enzymes, and thus presented single band with the length of 239 bp on the gel map (Figure 2B), while PPARD -87 T>C with T/C type was partially digested by BslI enzymes, presenting two bands with the length of 239 bp and 203 bp (Figure 2C). Besides, PPARD -87 T>C with C/C type only showed one band with the length of 203 bp due to completely digestion by BslI enzymes (Figure 2D).