LAUSR

Low effective conventional therapies were used to achieve survival results in mortality associated with breast cancer. Organo-copper compounds exerted cytotoxic activity against variety of cancer cell lines. In our study, the antiproliferative effect of SCP against MCF-7 and MDA-MB-231 cell lines, which differed in hormone receptor, was examined using MTT assay. To examine the possible relation between the cytotoxic activity of SCP and oxidative stress, we measured ROS levels in both human breast cell lines. Changes in apoptotic gene expression were detected by RT-PCR confirmed using western blot analysis. We found that SCP manifested anti-proliferative activities in both MCF-7 cells and MDA-MB-231 cells. The antitumor activity of SCP was studied at concentrations of 50 and half IC50. Analysis revealed decreased Bcl2 and increased P53 mRNA, suggesting that this compound exerts its regulatory action on apoptotic genes at the level of transcription. Our results suggest that SCP-induced tumor cell apoptosis get back to activation of both caspase-3 and caspase-9 regulated pathways. In addition, apoptotic features were found at morphological observation. The antitumor effect of SCP can further be enhanced by use of combination therapy and novel drug delivery systems, thus making it a promising candidate for management of breast cancer patients.