LAUSR

Objectives Plant-mediated synthesis [silver (Ag) to form Ag nanoparticles (AgNPs)] is becoming progressively well accepted in many scientific and pharmaceutical fields. The aim of this study was to synthesize AgNPs using air-dried leaves of four neglected vegetables, i.e. Ceratotheca sesamoides, Ceiba pentandra, Crassocephalum crepidioides, and Launaea taraxacifolia. Materials and Methods Ultraviolet-visible (UV-Vis) spectroscopy, fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) were used for characterization. Cell stabilization membrane and lipoxidase assays were used to determine used to assess the antiinflammatory activities while 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess the antioxidant activities of AgNPs [L. taraxacifolia-AgNPs, C. sesamoides Ag nanoparticles (CS-AgNPs), C. pentandra Ag nanoparticles (CP-AgNPs), and C. crepidioides AgNPs (CC-AgNPs)]. Results The UV-Vis spectra of the synthesized NPs displayed absorption bands at around 360-440 nm, which is a characteristic band for AgNPs. The SEM image showed that the AgNPs formed were spherical in morphology. CC-AgNPs exhibited the most significant inhibitory activity against human red blood cell membrane stabilizasyonu [median inhibitory concentration (IC50): 32.2 μg/mL] while CS-AgNPs displayed the most significant inhibitory activity against lipoxygenases (IC50: 32.8 μg/mL). CP-AgNPs exhibited the most significant antioxidant effect against both ABTS and DPPH (IC50: 5.5 and 6.4 μg/mL) when compared to ascorbic acid (IC50: 4.7 μg/mL). Conclusion The synthesized AgNPs were found to be stable and the FTIR evidence suggested that the phytochemicals in the vegetables might have played an important role in the reduction and stabilization of AgNPs. This work showed that the synthesized AgNPs from non-cultivated vegetables can find relevance and application in health, drugs, food and environmental science. The evidences herein further confirmed their ethnopharmacological applications.