OBJECTIVE To determine the reference interval of biotinidase activity in healthy neonates. METHODS The cross-sectional study was conducted at the Department of Chemical Pathology and Endocrinology, Armed Forces Institute of… Click to show full abstract
OBJECTIVE To determine the reference interval of biotinidase activity in healthy neonates. METHODS The cross-sectional study was conducted at the Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan, from May to November 2019, and comprised blood samples collected from healthy neonates aged 2-6 days. The samples were collected on filter paper and analysed on genetic screening processor based on dissociation-enhanced lanthanide flouroimmunoassay. Data was analysed using SPSS 21. RESULTS Of the 120 dried blood spot specimens, 81(67.5%) were from male babies and 39(32.5%) from female babies. Reference interval for biotinidase activity, based on 2.5th and 97.5th percentiles, was from 3.0 to 11.0 nmol/ml/min. CONCLUSIONS Screening of newborns for biotinidase deficiency is crucial to prevent irreversible neurological damage.
Journal Title: JPMA. The Journal of the Pakistan Medical Association
Year Published: 2022
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