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Preparation and Characterization of Chitosan from Perna viridis (Linnaeus, 1758) shell waste as raw material

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Chitosan is extracted from Perna viridis as the staring source via the cycle of chitin deacetylation, which is conducted at 90°C for 6 hours using 40% NaOH. Physiochemical properties such… Click to show full abstract

Chitosan is extracted from Perna viridis as the staring source via the cycle of chitin deacetylation, which is conducted at 90°C for 6 hours using 40% NaOH. Physiochemical properties such as yield (18%), ash content (0.626%), moisture content (2.9%), and solubility, degree of deacetylation (55), fat binding capability (209%) and water binding ability (254 %) revealed that P.viridis is an important alternative source of chitosan. Fourier transforms infrared spectroscopy (FTIR) analysis showed the characteristic peaks of OH at 3400cm-1 and amine at 1660cm-1, the X-ray diffraction (XRD) analysis suggested two critical characteristic peaks at 18° and 34° at (2θ). Scanning electron microscope (SEM) was used to evaluate the surface morphology of isolated chitosan. Thermogravimetric analysis (TG/DTA) was also used to describe the thermal stability of P.viridis chitosan. The procoagulant capacity, plasma recalcification time assays and minimal bactericidal activity verified the hemocompatibility and antibacterial activity of the preparation of chitosan.

Keywords: preparation characterization; characterization chitosan; perna viridis; viridis; chitosan

Journal Title: Research Journal of Pharmacy and Technology
Year Published: 2021

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