Considering the importance of bean cultivation, the objective was to prove the effectiveness of the seed microbiolization method in the control of Sclerotinia sclerotiorum ( Ss ) on black bean… Click to show full abstract
Considering the importance of bean cultivation, the objective was to prove the effectiveness of the seed microbiolization method in the control of Sclerotinia sclerotiorum ( Ss ) on black bean seeds (cv. IPR Tuiuiu), inoculated by the water restriction method. The treatments were fungicide methyl thiophanate + fluazinam (350.0 g L -1 + 52.5 g L -1 ); Trichoderma asperellum BV-10 (1.0 x 10 10 viable conidia mL -1 ); T. harzianum strain CCT 7589 (1 x 10 9 CFUs L -1 ); Bacillus subtilis BV-02 (minimum 3.0 x 10 9 CFU mL -1 ); B. amyloliquefaciens isolated BV03 (minimum 3.0 x 10 9 CFU L -1 ); Positive control (seeds exposed to Ss ); Negative control (NC) + fungicide; NC + T. asperellum ; NC + T. harzianum ; NC + B. subtilis ; NC + B. subtilis ; and NC (PDA medium plus restricting). To verify the effects on germination, vigor, and the health of bean seeds, and seedling emergence in the field. Microbiolization with T. asperellum and T. harzianum , provides improvement in germination, vigor, and health, but is not superior to fungicide treatment. Bacillus subtilis and B. amyloliquefaciens , in seeds not inoculated with the pathogen, produce the best results for green and dry mass (g). These may be used in the integrated management of white bean mold.
               
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