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Expression of Heat-labile Enterotoxin of Escherichia coli in Biolistic Transformed Hairy Roots of Daucus carota L

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Production of antigens and antibodies in plant provide a potential approach for the development of vaccines for oral delivery.1 Traditional vaccines are facing challenges like storage, distribution and administration but… Click to show full abstract

Production of antigens and antibodies in plant provide a potential approach for the development of vaccines for oral delivery.1 Traditional vaccines are facing challenges like storage, distribution and administration but recently their safety and effectiveness has also raise many issues.2 Expression and secretion of recombination proteins, antigens and antibodies through transgenic hairy roots in confined media under controlled environment have opened door to simple, feasible and economic option compared to animal and bacterial systems.3,4 Hairy roots are non-photosynthetic and rapidly grow in simple culture media without requirement of phytohormones. Easy genetic manipulation, low-cost downstream purification and easy scaleup in bioreactors attract researchers to hairy roots.5

Keywords: expression heat; hairy roots; escherichia coli; enterotoxin escherichia; heat labile; labile enterotoxin

Journal Title: Polymer Journal
Year Published: 2019

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