LAUSR

Probiotics are live microbes, which have the health promoting factors and they are naturally occurring in various kinds of food. During the recent period, the promising interest in naturally fermented food due to its benefits for its richest nutrients and biological property known as probiotic organism, thus we had taken toddy as one of its kind. Toddy is naturally fermented by tapping the male inflorescence of coconut tree. A total of 43 toddy samples were taken, among which 137 organisms were isolated and after the screening for its probiotic nature, one organism was screened out and it was identified by 16S rRNA sequence analysis which confirmed the organism as Lactobacillus fermentum KT183369, and it was further subjected for its application as probiotics. The organism was identified for its antioxidant activity and also for exhibiting the adhesive property into the human beings in order to adhere to the host epithelial and thus was checked using CaCo2 and HT29 cell line assay.