LAUSR

Teicoplanin is an antibiotic belonging to glycopeptides. A selective, specific and sensitive stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Teicoplanin in tablet dosage forms. Reversed-phase chromatography was performed on Shimadzu Model UFLC system equipped with PDA detector using Agilent column (150 mm × 4.6 mm i.d., 3.5 µm particle size) and mobile phase consisting of tetra butyl ammonium hydrogen sulphate and acetonitrile mixture (47:53, v/v) with flow rate 0.8 ml/min. Linearity was observed in the concentration range of 0.5-200 µg/ml with regression equation y = 115528x + 48907 (R2 = 0.9997). For precision and accuracy studies, relative standard deviation was <2%. Teicoplanin was subjected to stress conditions of degradation in aqueous solutions including acidic, alkaline, oxidation, photolysis and thermal degradation. The developed method was validated with regard to linearity, accuracy, precision, selectivity and robustness and the method was found to be precise, accurate, linear and specific.