LAUSR

Objectives: The purpose of this study was to prepare and evaluate the chitosan-tripolyphosphate (CS-TPP) nanoparticles suspension as an antibacterial agent. Material and Methods: CS-TPP nanoparticles suspension were prepared by ionic gelation method with various concentrations of CS and TPP. The prepared nanoparticles suspension were evaluated for particle size, sedimentation, interaction between CS and TPP, particles morphology, and antibacterial activity against Staphylococcus aureus (Gram positive) and Pseudomonas aeruginosa (Gram negative). Results: The results showed that among all of the concentrations used, the formulation of 0.25% CS and 0.1% sodium TPP resulted the smallest particles size (average diameter was 238.17 nm) with spherical morphology, no sedimentation after centrifugation at 6000 rpm for 2 hours, and gave the highest antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Conclusion: CS-TPP nanoparticles can be formed by using at certain concentration of CS and TPP solution and the CS-TPP nanoparticles suspension have an antibacterial activity.