LAUSR

Background Our study evaluated how soil organic carbon (SOC) and soil inorganic carbon (SIC) recovered over time in deep loessial soil as overgrazed grassland was fenced and restored. Methods The study was conducted in the Yunwu Mountain Nature Reserve in the Ningxia Autonomous Region of China. In it we compared soil data from three grazed grassland (G) sites, three sites that were fenced for 15 years (F15), and three sites that were fenced for 30 years (F30) as a so-called ‘space for time series’. Results and Discussion We compared SOC accumulation in soil up to 200 cm below the surface in G, F15, and F30 plots. An increase in SOC correlated with a decrease in soil pH, and decreased soil bulk density. However, SOC sequestration in fenced plots was largely offset by a decrease in SIC, which was closely correlated (r = 0.713, p = 0.001) with SOC-driven soil pH decline. We observed no significant increase in soil total carbon in the F15 or F30 sites after comparing them to G. Conclusions Our data indicate that fencing causes the slow diffusion processes to intensify the soil property changes from increased litter return, and this slow diffusion process is still active 30 years after fencing at 100–200 cm soil depths in the studied deep loessial soil. These findings are likely applicable to similar sites.