Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.
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DOI: 10.1007/978-1-4939-9121-1_3
Abstract: Escherichia coli (E. coli) is the most widely used expression host for recombinant proteins due to high expression yields and straightforward molecular cloning. Directed evolution of G protein-coupled receptors (GPCRs) has made several of these… read more here.
Keywords: neurotensin receptor; solution nmr; methionine; 13ch3 methionine ... See more keywords