Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S.
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DOI: 10.1111/1462-2920.15553
Abstract: Universal primers for SSU rRNA genes allow profiling of natural communities by simultaneously amplifying templates from Bacteria, Archaea, and Eukaryota in a single PCR reaction. Despite the potential to show relative abundance for all rRNA… read more here.
Keywords: rrna; mock; universal primers; pcr ... See more keywords