Beyond sequencing: fast and easy microbiome profiling by flow cytometry
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Toxicology"
DOI: 10.1007/s00204-019-02527-1
Abstract: Analyses of the microbiome are increasingly important to understand the impact of the microbiota on physiological functions, diseases, and toxicity in the host organism. The microbiome is the sum of all bacteria, viruses, fungi, and… read more here.
Keywords: 16s rdna; whole genome; rdna rrna; beyond sequencing ... See more keywords
Genetic diversity of Haemaphysalis qinghaiensis (Acari: Ixodidae) in western China
Sign Up to like & getrecommendations! Published in 2018 at "Experimental and Applied Acarology"
DOI: 10.1007/s10493-018-0242-2
Abstract: Haemaphysalis qinghaiensis as an endemic species in China mainly infests domestic animals and causes great harm to animals and humans in Northwestern plateau. However, there is no information about genetic diversity within the recently established… read more here.
Keywords: haemaphysalis qinghaiensis; 16s rdna; qinghaiensis; genetic diversity ... See more keywords
Establishment of a method for measuring leukocyte phagocytosis based on 16S rDNA
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biology Reports"
DOI: 10.1007/s11033-018-4538-4
Abstract: The goal of this study was to develop a method for measuring leukocyte phagocytosis based on 16S rDNA, and to investigate its clinical significance. Whole blood was collected and incubated with bacteria. Free bacteria were… read more here.
Keywords: 16s rdna; microscopy; leukocyte phagocytosis; method ... See more keywords
Amplification of 16S rDNA reveals important fish mislabeling in Madrid restaurants
Sign Up to like & getrecommendations! Published in 2019 at "Food Control"
DOI: 10.1016/j.foodcont.2018.09.020
Abstract: Abstract Food fraud encompasses economic fraud and can produce health problems for consumers, as well as conservation problems for the involved species. Nevertheless, few studies tested for mislabeling in restaurants. In this study, we tested… read more here.
Keywords: amplification 16s; rdna reveals; rdna; reveals important ... See more keywords
Prediction of functional metagenomic composition using archived 16S rDNA sequence data from the gut microbiota of livestock
Sign Up to like & getrecommendations! Published in 2018 at "Livestock Science"
DOI: 10.1016/j.livsci.2018.04.017
Abstract: Abstract The volume of microbial genetic terabases in public repositories is getting infamous, yet much of this data has been used only once. On top of this global concern, there is still much to be… read more here.
Keywords: rdna sequence; 16s rdna; livestock; sequence data ... See more keywords
Effects of sampling and storage procedures on 16S rDNA amplicon sequencing results of kelp microbiomes.
Sign Up to like & getrecommendations! Published in 2022 at "Marine genomics"
DOI: 10.1016/j.margen.2022.100944
Abstract: Brown macroalgae, including the kelp Saccharina latissima, are of both ecological and increasing economic interest. Together with their microbiota, these organisms form a singular entity, the holobiont. Sampling campaigns are required to study the microbiome… read more here.
Keywords: effects sampling; storage procedures; procedures 16s; sampling storage ... See more keywords
Wastewater discharge drives ARGs spread in the coastal area: A case study in Hangzhou Bay, China.
Sign Up to like & getrecommendations! Published in 2020 at "Marine pollution bulletin"
DOI: 10.1016/j.marpolbul.2019.110856
Abstract: The distribution of 14 ARGs, intI1, and 16S rDNA were analysed in 4 wastewater treatment plants (WWTPs), 2 effluent receiving areas (ERAs), and Hangzhou Bay (HZB). The results showed that each integrated WWTP (IWWTP) received… read more here.
Keywords: drives args; hangzhou bay; 16s rdna; discharge drives ... See more keywords
Comparison of MALDI-TOF MS Biotyper and 16S rDNA sequencing for the identification of Pseudomonas species isolated from fish.
Sign Up to like & getrecommendations! Published in 2019 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2019.04.024
Abstract: Effective and reliable methods of identification of Pseudomonas species are important for the characterization of microorganisms. Freshwater ecosystems are an important source of Pseudomonas species, including those pathogenic to fish and humans. The aim of… read more here.
Keywords: 16s rdna; pseudomonas; identification pseudomonas; identification ... See more keywords
Determination of uterine bacterial community in postpartum dairy cows with metritis based on 16S rDNA sequencing
Sign Up to like & getrecommendations! Published in 2020 at "Veterinary and Animal Science"
DOI: 10.1016/j.vas.2020.100102
Abstract: Metritis is a frequently occurring diseases in postpartum cows and is one of the important reasons for the infertility of dairy cows, accounting for 20–30% of dairy cow diseases and has serious implications for the… read more here.
Keywords: 16s rdna; dairy cows; healthy cows; cows metritis ... See more keywords
Programmable T-Junction Structure-Assisted CRISPR/Cas12a Electrochemiluminescence Biosensor for Detection of Sa-16S rDNA.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c18930
Abstract: Herein, a strand displacement amplification (SDA)-assisted CRISPR/Cas12a (LbCpf1) electrochemiluminescence (ECL) biosensor was fabricated for ultrasensitive identification of Staphylococcus aureus (Sa)-16S rDNA. A porphyrinic Zr metal-organic framework (MOF) (PCN-224) nanomaterial was prepared as the coreactant accelerator,… read more here.
Keywords: 16s rdna; structure; rdna; assisted crispr ... See more keywords
Exclusive use of digital PCR allows an absolute assay of heat-killed Lactobacilli in foods targeting multiple copies of 16S rDNA
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-69206-5
Abstract: The real-time PCR (qPCR) and digital PCR (dPCR) to amplify a single-copy of house-keeping genes (i.e., hsp60, pheS or tuf) are used for the assay of limited microbial species. In general, with a single-copy gene,… read more here.
Keywords: 16s rdna; heat killed; copies 16s; rdna ... See more keywords