Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12590
Abstract: Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene amplicon sequencing has proven to be a powerful strategy for diagnosing bacterial… read more here.
Keywords: identification; 16s rrna; minion; direct pcr ... See more keywords
Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus‐specific primers
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DOI: 10.1002/mbo3.1274
Abstract: The goal of this study was to design genus‐specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon‐based sequencing of the 16S rRNA gene in fish‐related samples and surrounding water. Efficient… read more here.
Keywords: bacterial genera; specific primers; 16s rrna; genus specific ... See more keywords
DMclust, a Density‐based Modularity Method for Accurate OTU Picking of 16S rRNA Sequences
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DOI: 10.1002/minf.201600059
Abstract: Clustering 16S rRNA sequences into operational taxonomic units (OTUs) is a crucial step in analyzing metagenomic data. Although many methods have been developed, how to obtain an appropriate balance between clustering accuracy and computational efficiency… read more here.
Keywords: rrna sequences; method; 16s rrna; density based ... See more keywords
High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Ecology"
DOI: 10.1007/s00248-019-01321-x
Abstract: Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of… read more here.
Keywords: 16s rrna; throughput sequencing; rrna gene; high throughput ... See more keywords
Analysis of Bacterial Communities by 16S rRNA Gene Sequencing in a Melon-Producing Agro-environment.
Sign Up to like & getrecommendations! Published in 2021 at "Microbial ecology"
DOI: 10.1007/s00248-021-01709-8
Abstract: Cantaloupe melons, which have been responsible of an increasing number of foodborne disease outbreaks, may become contaminated with microbial pathogens during production. However, little information is available on the microbial populations in the cantaloupe farm… read more here.
Keywords: analysis bacterial; analysis; bacterial communities; communities 16s ... See more keywords
Retrieved 16S rRNA and nifH sequences reveal co-dominance of Bradyrhizobium and Ensifer (Sinorhizobium) strains in field-collected root nodules of the promiscuous host Vigna radiata (L.) R. Wilczek
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8609-6
Abstract: In the present study, the relative distribution of endophytic rhizobia in field-collected root nodules of the promiscuous host mung bean was investigated by sequencing of 16S ribosomal RNA (rRNA) and nifH genes, amplified directly from… read more here.
Keywords: nifh sequences; bradyrhizobium ensifer; 16s rrna; host ... See more keywords
Novel primers for 16S rRNA gene-based archaeal and bacterial community analysis in oceanic trench sediments.
Sign Up to like & getrecommendations! Published in 2022 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-022-11893-3
Abstract: High-throughput sequencing of the 16S ribosomal RNA (16S rRNA) gene has been successfully applied to explore the microbial structure and dynamics in various environments. The distinctive microbial communities in oceanic trench sediments are expected because… read more here.
Keywords: trench sediments; rrna gene; novel primers; 16s rrna ... See more keywords
Comparison of Cell Culture with Three Conventional Polymerase Chain Reactions for Detecting Chlamydophila pneumoniae in Adult’s Pharyngotonsillitis
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DOI: 10.1007/s00284-020-02106-z
Abstract: Chlamydophila pneumoniae is an intracellular pathogen responsible for respiratory tract infections. The isolation of the microorganism from clinical specimens is essential for a diagnosis. However, the identification of C. pneumoniae by cell cultures is very… read more here.
Keywords: chlamydophila pneumoniae; pneumoniae; 16s rrna; gene ... See more keywords
Letter to the Editor concerning “Bacteria: back pain, leg pain and Modic sign—a surgical multicentre comparative study” by Fritzell P, et al. (Eur Spine J [2019]; doi:10.1007/s00586-019-06164-1)
Sign Up to like & getrecommendations! Published in 2020 at "European Spine Journal"
DOI: 10.1007/s00586-020-06318-6
Abstract: We read with great interest the recent article on bacterial presence in intervertebral disc samples by Fritzell et al. The authors have performed a multicenter analysis exploring the correlation of bacterial presence/growth in discs and… read more here.
Keywords: methodology; dna; analysis; 16s rrna ... See more keywords
Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents
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DOI: 10.1007/s10123-018-00038-0
Abstract: This study aimed to analyze the bacterial diversity in carwash effluents and to determine their potential for use in microbial degradation of environmental contaminants. Nine carwash effluent samples were collected for physicochemical and bacterial community… read more here.
Keywords: rrna amplicon; targeted 16s; analysis; carwash effluents ... See more keywords
Composition, diversity and abundance of Candidatus M. oxyfera-like bacteria in response to the estuary salinity gradient
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DOI: 10.1007/s10533-019-00545-w
Abstract: Abstract“Candidatus Methylomirabilis oxyfera” (M. oxyfera) bacteria has attracted much attention recently since it plays an important role in performing methane oxidation. Most studies on M. oxyfera-like bacteria have been carried out in freshwater ecosystems, however… read more here.
Keywords: oxyfera like; like bacteria; 16s rrna; salinity ... See more keywords