Sequencing-based detection of 23S rRNA domain V mutations in treatment-naïve Helicobacter pylori patients from Malaysia.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of global antimicrobial resistance"
DOI: 10.1016/j.jgar.2020.10.012
Abstract: OBJECTIVES In Malaysia, the prevalence ofHelicobacter pylori resistance towards clarithromycin is increasing. This study aims to determine the mutations in the 23S rRNA domain V directly using bacterial DNA extracted from gastric biopsy specimens with… read more here.
Keywords: rrna domain; treatment; 23s rrna; pylori ... See more keywords
Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-03458-6
Abstract: The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species… read more here.
Keywords: 16s 23s; identification; rrna region; culture ... See more keywords
µgreen-db: a reference database for the 23S rRNA gene of eukaryotic plastids and cyanobacteria
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-62555-1
Abstract: Studying the ecology of photosynthetic microeukaryotes and prokaryotic cyanobacterial communities requires molecular tools to complement morphological observations. These tools rely on specific genetic markers and require the development of specialised databases to achieve taxonomic assignment.… read more here.
Keywords: rrna gene; reference database; 23s rrna;
Mutations in Genes Encoding 23S rRNA and FadD32 May be Associated with Linezolid Resistance in Mycobacteroides abscessus.
Sign Up to like & getrecommendations! Published in 2023 at "Microbial drug resistance"
DOI: 10.1089/mdr.2022.0068
Abstract: Linezolid is one of the antibiotics used to treat the Mycobacteroides abscessus infection. However, linezolid-resistance mechanisms of this organism are not well understood. The objective of this study was to identify possible linezolid-resistance determinants in… read more here.
Keywords: mycobacteroides abscessus; linezolid resistance; resistance; 23s rrna ... See more keywords
Chlamydia trachomatis variants escaping detection in the Aptima Combo 2® assay in the United States.
Sign Up to like & getrecommendations! Published in 2022 at "Sexually transmitted diseases"
DOI: 10.1097/olq.0000000000001617
Abstract: BACKGROUND The Aptima Combo 2® (AC2) assay manufactured by Hologic, Inc. detects Neisseria gonorrhoeae (NG) and/or Chlamydia trachomatis (CT) in urogenital and extragenital specimens by targeting either a 16S rRNA (NG) or 23S rRNA (CT)… read more here.
Keywords: aptima combo; ac2 assay; chlamydia trachomatis; 23s rrna ... See more keywords
Confident phylogenetic identification of uncultured prokaryotes through long read amplicon sequencing of the 16S‐ITS‐23S rRNA operon
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Microbiology"
DOI: 10.1111/1462-2920.14636
Abstract: Summary Amplicon sequencing of the 16S rRNA gene is the predominant method to quantify microbial compositions and to discover novel lineages. However, traditional short amplicons often do not contain enough information to confidently resolve their… read more here.
Keywords: rrna operon; rrna gene; 23s rrna; rrna ... See more keywords
Persister Cells Resuscitate via Ribosome Modification by 23S rRNA Pseudouridine Synthase RluD.
Sign Up to like & getrecommendations! Published in 2019 at "Environmental microbiology"
DOI: 10.1111/1462-2920.14828
Abstract: Upon a wide range of stress conditions (e.g., nutrient, antibiotic, oxidative), a subpopulation of bacterial cells known as persisters survive by halting metabolism. These cells resuscitate rapidly to reconstitute infections once the stress is removed… read more here.
Keywords: persister cells; rrna pseudouridine; cells resuscitate; rlud ... See more keywords
Atypical Mutation in Neisseria gonorrhoeae 23S rRNA Associated with High-Level Azithromycin Resistance
Sign Up to like & getrecommendations! Published in 2020 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.00885-20
Abstract: A2059G mutation in the 23S rRNA gene is the only reported mechanism conferring high-level azithromycin resistance (HL-AZMR) in Neisseria gonorrhoeae. Through U.S. gonococcal antimicrobial resistance surveillance projects, we identified four HL-AZMR gonococcal isolates lacking this… read more here.
Keywords: resistance; 23s rrna; level azithromycin; high level ... See more keywords
O05.4 Does high-level azithromycin resistance emerge from low-level resistance in neisseria gonorrhoeae?
Sign Up to like & getrecommendations! Published in 2017 at "Sexually Transmitted Infections"
DOI: 10.1136/sextrans-2017-053264.27
Abstract: Introduction High-level azithromycin (Azi) resistance (HL-AziR) threatens gonorrhoea dual therapy (ceftriaxone 500 mg and Azi 1g) as it renders Azi ineffective. Between November 2014–2016, 58 cases of HL-AziR (MIC >256 mg/L) N. gonorrhoeae (NG) were detected in… read more here.
Keywords: st9768; azir; level; resistance ... See more keywords
Development of a reference data set for assigning Streptococcus and Enterococcus species based on next generation sequencing of the 16S–23S rRNA region
Sign Up to like & getrecommendations! Published in 2019 at "Antimicrobial Resistance and Infection Control"
DOI: 10.1186/s13756-019-0622-3
Abstract: BackgroundMany members of Streptococcus and Enterococcus genera are clinically relevant opportunistic pathogens warranting accurate and rapid identification for targeted therapy. Currently, the developed method based on next generation sequencing (NGS) of the 16S–23S rRNA region… read more here.
Keywords: 16s 23s; reference; identification; rrna region ... See more keywords
The Bacillus subtilis open reading frame ysgA encodes the SPOUT methyltransferase RlmP forming 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA.
Sign Up to like & getrecommendations! Published in 2022 at "RNA"
DOI: 10.1261/rna.079131.122
Abstract: A previous bioinformatic analysis predicted that the ysgA open reading frame of Bacillus subtilis encodes an RNA methyltransferase of the SPOUT superfamily. Here we show that YsgA is the 2'-O-methyltransferase that targets position G2553 (Escherichia… read more here.
Keywords: reading frame; ysga; 23s rrna; methyltransferase ... See more keywords