Pulse-Chase Labeling of Protein Antigens with [35S]Methionine.
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DOI: 10.1101/pdb.prot098525
Abstract: Pulse-chase labeling of antigens with [35S]methionine is used to determine the relative half-life of a protein. In this protocol, intracellular unlabeled methionine levels are reduced by starvation of cells for 0.5-1 h, and then the… read more here.
Keywords: methionine; pulse chase; 35s methionine; chase labeling ... See more keywords