Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201800523
Abstract: A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE‐LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as… read more here.
Keywords: detection; 5hmc levels; detection hydroxymethylcytosine; hydroxymethylation ... See more keywords
A Bayesian hierarchical model for improving measurement of 5mC and 5hmC levels: Toward revealing associations between phenotypes and methylation states
Sign Up to like & getrecommendations! Published in 2022 at "Genetic Epidemiology"
DOI: 10.1002/gepi.22489
Abstract: 5‐hydroxymethylcytosine (5hmC) is a methylation state linked with gene regulation, commonly found in cells of the central nervous system. 5hmC is associated with demethylation of cytosines from 5‐methylcytosine (5mC) to the unmethylated state. The presence… read more here.
Keywords: hierarchical model; methylation; methylation states; 5hmc levels ... See more keywords
Epigenetic downregulation of TET3 reduces genome‐wide 5hmC levels and promotes glioblastoma tumorigenesis
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DOI: 10.1002/ijc.32520
Abstract: Loss of 5‐hydroxymethylcytosine (5hmC) has been associated with mutations of the ten–eleven translocation (TET) enzymes in several types of cancer. However, tumors with wild‐type TET genes can also display low 5hmC levels, suggesting that other… read more here.
Keywords: genome wide; 5hmc levels; epigenetic downregulation; glioblastoma tumorigenesis ... See more keywords
ELISA-Based Quantitation of Global 5hmC Levels.
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DOI: 10.1007/978-1-0716-1294-1_4
Abstract: 5-Hydroxymethylcytosine (5hmC) is an abundant DNA modification in human and mouse brain, as well as in embryonic stem cells, while severely depleted in multiple types of cancer. Assays for 5hmC detection and quantification, both on… read more here.
Keywords: based quantitation; elisa based; detection; 5hmc levels ... See more keywords