The quest for absolute abundance: The use of internal standards for DNA‐based community ecology
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Ecology Resources"
DOI: 10.1111/1755-0998.13247
Abstract: To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no… read more here.
Keywords: dna; absolute abundance; ecology; quest absolute ... See more keywords
A human blood–arachnoid barrier atlas of transporters, receptors, enzymes, and tight junction and marker proteins: Comparison with dog and pig in absolute abundance
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Neurochemistry"
DOI: 10.1111/jnc.15599
Abstract: The purpose of this study was to elucidate the absolute abundance of transporters, enzymes, receptors, and tight junction and marker proteins at human blood–arachnoid barrier (BAB) and compare with those of dogs and pigs. Protein… read more here.
Keywords: dogs pigs; absolute abundance; tight junction; marker proteins ... See more keywords
Vital statistics, absolute abundance and preservation rate of Tyrannosaurus rex
Sign Up to like & getrecommendations! Published in 2023 at "Palaeontology"
DOI: 10.1111/pala.12648
Abstract: I present a simulation model on vital statistics, absolute abundance (N, total number of individuals that ever lived) and preservation rate (p, minimum number of fossils known divided by N) of Tyrannosaurus rex. It is… read more here.
Keywords: absolute abundance; vital statistics; rex; preservation rate ... See more keywords
Gradient Internal Standard Method for Absolute Quantification of Microbial Amplicon Sequencing Data
Sign Up to like & getrecommendations! Published in 2021 at "mSystems"
DOI: 10.1128/msystems.00964-20
Abstract: To solve the problem of amplicon sequencing cannot discern the microbiota absolute abundance, we proposed a gradient internal standard absolute quantification method. We used Chinese liquor fermentation as a model system to demonstrate the reliability… read more here.
Keywords: abundance; method; amplicon sequencing; gradient internal ... See more keywords
Absolute quantitation of microbiota abundance in environmental samples
Sign Up to like & getrecommendations! Published in 2018 at "Microbiome"
DOI: 10.1186/s40168-018-0491-7
Abstract: BackgroundMicrobial communities (microbiota) influence human and animal disease and immunity, geochemical nutrient cycling and plant productivity. Specific groups, including bacteria, archaea, eukaryotes or fungi, are amplified by PCR to assess the relative abundance of sub-groups… read more here.
Keywords: absolute quantitation; absolute abundance; microbiota abundance; abundance ... See more keywords
Relative and Quantitative Rhizosphere Microbiome Profiling Results in Distinct Abundance Patterns
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.798023
Abstract: Next-generation sequencing is one of the most popular and cost-effective ways of characterizing microbiome in multiple samples. However, most of the currently available amplicon sequencing approaches are limited, as they result in relative abundance profiles… read more here.
Keywords: absolute abundance; abundance; estimated absolute; relative abundance ... See more keywords