Absolute Protein Quantification by Mass Spectrometry: Not as Simple as Advertised.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00858
Abstract: Stable isotopically labeled (SIL) tryptic peptides, cleavable SIL peptides, and a full-length SIL protein were compared for internal calibration (i.e., as internal calibrators) and external calibration (i.e., as internal standards) when quantifying three forms of… read more here.
Keywords: protein; sil; absolute protein; digestion ... See more keywords
Artificial Intelligence Understands Peptide Observability and Assists With Absolute Protein Quantification
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2018.01559
Abstract: Targeted mass spectrometry has become the method of choice to gain absolute quantification information of high quality, which is essential for a quantitative understanding of biological systems. However, the design of absolute protein quantification assays… read more here.
Keywords: peptide observability; protein quantification; absolute protein; quantification ... See more keywords