Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2277-3_1
Abstract: Cell signaling governs the basic functions of cells by molecular interactions that involve of many proteins. The abundance of signaling proteins can directly influence cellular responses to external signal, contributing to cellular heterogeneity. Absolute quantification… read more here.
Keywords: using quantitative; absolute quantification; proteins using; signaling proteins ... See more keywords
Absolute quantification of targeted meat and allergenic protein additive peptide markers in meat products.
Sign Up to like & getrecommendations! Published in 2019 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.08.131
Abstract: We present an implementation of the absolute quantification (AQUA) method for monitoring of peptide abundance in complex mixtures of processed proteins. Specific peptide markers from meats (chicken, duck, goose, pork and beef) and common protein… read more here.
Keywords: absolute quantification; protein; quantification targeted; peptide markers ... See more keywords
Versatile and multiplexed mass spectrometry-based absolute quantification with cell-free-synthesized internal standard peptides.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2021.104393
Abstract: Preparation of stable isotope-labeled internal standard peptides is crucial for mass spectrometry (MS)-based targeted proteomics. Herein, we developed versatile and multiplexed absolute protein quantification method using MS. A previously developed method based on the cell-free… read more here.
Keywords: absolute quantification; internal standard; cell free; quantification ... See more keywords
Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2017.03.003
Abstract: Highlights • First protocol for absolute quantification of cassava brown streak viruses.• Standard templates for specific absolute quantification of CBSVs generated.• Acceptable standard curves for specific absolute quantification of CBSVs prepared.• Screening efficiency for CBSD-resistance… read more here.
Keywords: absolute quantification; quantification cassava; brown streak; cassava brown ... See more keywords
Fully integrated protein absolute quantification platform for analysis of multiple tumor markers in human plasma.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122102
Abstract: In this study, we developed a fully integrated protein absolute quantification platform for simultaneous analysis of multiple tumor markers in human plasma, by which multiple target proteins (alpha-fetoprotein, prostate-specific antigen, carcino-embryonic antigen and mucin-1) were… read more here.
Keywords: absolute quantification; tumor markers; analysis; human plasma ... See more keywords
Streamlined Subclass-Specific Absolute Quantification of Serum IgG Glycopeptides Using Synthetic Isotope-Labeled Standards.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c04462
Abstract: Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised… read more here.
Keywords: absolute quantification; igg glycopeptides; isotope labeled;
Intracellular Absolute Quantification of Oligonucleotide Therapeutics by NanoSIMS.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02111
Abstract: Antisense oligonucleotide (ASO)-based therapeutics hold great potential for the treatment of a variety of diseases. Therefore, a better understanding of cellular delivery, uptake, and trafficking mechanisms of ASOs is highly important for early-stage drug discovery.… read more here.
Keywords: quantification oligonucleotide; quantification; intracellular absolute; aso ... See more keywords
Particulate Standard Establishment for Absolute Quantification of Nanoparticles by LA-ICP-MS.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00028
Abstract: The development of nanotechnology has transformed many cutting-edge studies related to single-molecule analysis into nanoparticle (NP) detection with a single-NP sensitivity and ultrahigh resolution. While laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been… read more here.
Keywords: quantification; particulate standard; standard establishment; establishment absolute ... See more keywords
Monte Carlo Modeling-Based Digital Loop-Mediated Isothermal Amplification on a Spiral Chip for Absolute Quantification of Nucleic Acids.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00031
Abstract: Digital loop-mediated isothermal amplification (dLAMP) is an attractive approach for absolute quantification of nucleic acids with high sensitivity and selectivity. Theoretical and numerical analysis of dLAMP provides necessary guidance for the design and analysis of… read more here.
Keywords: absolute quantification; nucleic acids; quantification nucleic; spiral chip ... See more keywords
Absolute Quantification of Water in Microporous Solids with 1H Magic Angle Spinning NMR and Standard Addition.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b01653
Abstract: Zeolites are microporous materials driving industrial scale adsorption, ion exchange, and catalytic processes. Their water content dramatically impacts their properties, but its quantification with Karl Fisher titration or thermal gravimetric analysis is problematic. When standard… read more here.
Keywords: absolute quantification; angle spinning; water; standard addition ... See more keywords
Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03965
Abstract: Host cell proteins (HCP) are a major class of impurities derived from recombinant protein production processes. While HCP are usually monitored by ELISA, mass spectrometry (MS)-based approaches are emerging as promising orthogonal methods. Here, we… read more here.
Keywords: absolute quantification; data independent; independent acquisition; hcp ... See more keywords