Development and validation of a high resolving power absolute quantitative per‐ and polyfluoroalkyl substances method incorporating Skyline data processing
Sign Up to like & getrecommendations! Published in 2022 at "Rapid Communications in Mass Spectrometry"
DOI: 10.1002/rcm.9295
Abstract: Rationale The ability to perform absolute quantitation and non‐targeted analysis on a single mass spectrometry instrument would be advantageous to many researchers studying per‐ and polyfluoroalkyl substances (PFAS). High‐resolution accurate mass (HRAM) instrumentation (typically deployed… read more here.
Keywords: per polyfluoroalkyl; processing; data processing; polyfluoroalkyl substances ... See more keywords
Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7411-5_15
Abstract: The purification of a functional soluble protein from biological or in vitro expression systems can be problematic and the enrichment of a functional membrane protein for biochemical analyses can be a serious technical challenge. Recently… read more here.
Keywords: absolute quantitation; quantitation vitro; vitro expressed; kinetic parameters ... See more keywords
Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02709
Abstract: Proteomic absolute quantitation strategies mainly rely on the use of synthetic stable isotope-labeled peptides or proteins as internal standards, which are highly costly and time-consuming to synthesize. To circumvent this limitation, we recently developed a… read more here.
Keywords: deamidation; mass spectrometry; absolute quantitation; cms ... See more keywords
Standard-Free Bioanalytical Approach for Absolute Quantitation of Drug Metabolites Utilizing Biosynthesis of Reciprocal Radio and Stable Isotopologues and Its Application.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b01830
Abstract: The following work describes a combined enzymatic and bioanalytical method that permits absolute quantitation of metabolites in biological samples without the requirement for reference metabolite standards. This technique was exemplified using a radio (14C) isotopologue… read more here.
Keywords: stable isotopologues; absolute quantitation; radio; radio stable ... See more keywords
Characterization of the Immunomodulatory Mechanism of a Pleurotus eryngii Protein by Isobaric Tags for Relative and Absolute Quantitation Proteomics
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Agricultural and Food Chemistry"
DOI: 10.1021/acs.jafc.0c00219
Abstract: PEP 1b is a novel immunoregulatory protein isolated from Pleurotus eryngii, a popular edible mushroom. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) approach and bioin... read more here.
Keywords: pleurotus eryngii; isobaric tags; relative absolute; absolute quantitation ... See more keywords
Absolute Quantitation of N-Nitrosamines by Coulometric Mass Spectrometry without Using Standards.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00064
Abstract: Carcinogenic N-nitrosamines were recently found in the sartan family of drugs and caused many drug recalls. Both of their detection and quantification are therefore important. Methods reported for N-nitrosamine quantitation rely on the use of… read more here.
Keywords: coulometric mass; mass spectrometry; absolute quantitation; quantitation ... See more keywords
Absolute quantitation of microbiota abundance in environmental samples
Sign Up to like & getrecommendations! Published in 2018 at "Microbiome"
DOI: 10.1186/s40168-018-0491-7
Abstract: BackgroundMicrobial communities (microbiota) influence human and animal disease and immunity, geochemical nutrient cycling and plant productivity. Specific groups, including bacteria, archaea, eukaryotes or fungi, are amplified by PCR to assess the relative abundance of sub-groups… read more here.
Keywords: absolute quantitation; absolute abundance; microbiota abundance; abundance ... See more keywords
Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Psychiatry"
DOI: 10.3389/fpsyt.2022.577857
Abstract: Objectives Isobaric tags for relative and absolute quantitation (iTRAQ) is a proteomic investigation that could be utilized for rapid identification and quantification of proteins, which we would use to identify differentially expressed proteins in treatment… read more here.
Keywords: tags relative; relative absolute; isobaric tags; absolute quantitation ... See more keywords
Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.74153
Abstract: Despite an unprecedented global research effort on SARS-CoV-2, early replication events remain poorly understood. Given the clinical importance of emergent viral variants with increased transmission, there is an urgent need to understand the early stages… read more here.
Keywords: individual sars; replication; cov rna; absolute quantitation ... See more keywords