Highly accurate-single chromosomal complete genomes using IonTorrent and MinION sequencing of clinical pathogens.
Sign Up to like & getrecommendations! Published in 2019 at "Genomics"
DOI: 10.1016/j.ygeno.2019.04.006
Abstract: Oxford Nanopore MinION sequencing technology has been gaining immense importance in identification of pathogen and antimicrobial resistance, though with 10-15% error rate. Short read technologies generates high accurate genome but with multiple fragments of genome.… read more here.
Keywords: iontorrent minion; highly accurate; single chromosomal; minion ... See more keywords
MrHAMER yields highly accurate single molecule viral sequences enabling analysis of intra-host evolution
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab231
Abstract: Abstract Technical challenges remain in the sequencing of RNA viruses due to their high intra-host diversity. This bottleneck is particularly pronounced when interrogating long-range co-evolved genetic interactions given the read-length limitations of next-generation sequencing platforms.… read more here.
Keywords: single molecule; intra host; mrhamer yields; accurate single ... See more keywords