Nucleic acid amplification-free detection of DNA and RNA at ultralow concentration.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2021.07.022
Abstract: The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10-18 M) concentration and lower is reviewed. These low concentrations correspond roughly to the most clinically desirable detection range… read more here.
Keywords: detection; free detection; amplification free; acid amplification ... See more keywords
Three-in-One System Based on Multi-Path Nucleic Acid Amplification for Bioanalysis of Pre-miRNA/miRNA and Dicer Activity.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00432
Abstract: Today, a lot of attention is being paid to the pre-miRNAs/miRNAs or activity of Dicer due to their important functions in various physiological processes. Especially, the intrinsic relationship among these associated targets is of significant… read more here.
Keywords: bioanalysis; pre mirna; acid amplification; multi path ... See more keywords
Nucleic Acid Amplification by Template-Dominated Click Chemistry for Ultrasensitive DNA/RNA Detection on an Electrochemical Readout Platform.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05421
Abstract: As an enzyme-free exponential nucleic acid amplification method, the click chemistry-mediated ligation chain reaction (ccLCR) has shown great prospects in the molecular diagnosis. However, the current optics-based ccLCR is challenged by remarkable nonspecific amplification, severely… read more here.
Keywords: dna; click chemistry; chemistry; acid amplification ... See more keywords
Highly Stable and Sensitive Nucleic Acid Amplification and Cell-Phone-Based Readout.
Sign Up to like & getrecommendations! Published in 2017 at "ACS nano"
DOI: 10.1021/acsnano.6b08274
Abstract: Key challenges with point-of-care (POC) nucleic acid tests include achieving a low-cost, portable form factor, and stable readout, while also retaining the same robust standards of benchtop lab-based tests. We addressed two crucial aspects of… read more here.
Keywords: phone based; acid amplification; nucleic acid; readout ... See more keywords
Multifunctional Nanoparticle Platform for Surface Accumulative Nucleic Acid Amplification and Rapid Electrochemical Detection: An Application to Pathogenic Coronavirus
Sign Up to like & getrecommendations! Published in 2023 at "ACS Sensors"
DOI: 10.1021/acssensors.2c02512
Abstract: Of various molecular diagnostic assays, the real-time reverse transcription polymerase chain reaction is considered the gold standard for infection diagnosis, despite critical drawbacks that limit rapid detection and accessibility. To confront these issues, several nanoparticle-based… read more here.
Keywords: platform; acid amplification; amplification; detection ... See more keywords
Fractal-LAMP: Label-free analysis of fractal precipitate for digital loop-mediated isothermal nucleic acid amplification.
Sign Up to like & getrecommendations! Published in 2020 at "ACS sensors"
DOI: 10.1021/acssensors.9b01974
Abstract: Nucleic acid amplification assays including loop-mediated isothermal amplification (LAMP) are routinely used in diagnosing diseases and monitoring water and food quality. The results of amplification in these assays are commonly measured with an analog fluorescence… read more here.
Keywords: fractal lamp; label free; acid amplification; nucleic acid ... See more keywords
Monitoring nucleic acid amplification process using a UiO-66-NH2-based fluorescent sensor.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc03995h
Abstract: Herein, we developed a nucleic acid amplification process monitoring scheme by the use of UiO-66-NH2, in which pyrophosphate ion (PPi) released from the amplification can competitively coordinate with Zr to weaken the ligand-to-metal charge transfer… read more here.
Keywords: amplification process; uio nh2; acid amplification; amplification ... See more keywords
Faradaic-free electrokinetic nucleic acid amplification (E-NAAMP) using localized on-chip high frequency Joule heating.
Sign Up to like & getrecommendations! Published in 2022 at "Biomicrofluidics"
DOI: 10.1063/5.0072198
Abstract: We present a novel Faradaic reaction-free nucleic acid amplification (NAA) method for use with microscale liquid samples. Unlike previous Joule heating methods where the electrodes produce electrolysis gaseous by-products and require both the electrodes be… read more here.
Keywords: amplification; joule heating; acid amplification; nucleic acid ... See more keywords
Isothermal nucleic acid amplification technology for rapid detection of virus
Sign Up to like & getrecommendations! Published in 2022 at "Critical Reviews in Biotechnology"
DOI: 10.1080/07388551.2022.2030295
Abstract: Abstract While the research field and industrial market of in vitro diagnosis (IVD) thrived during and post the COVID-19 pandemic, the development of isothermal nucleic acid amplification test (INAAT) based rapid diagnosis was engendered in… read more here.
Keywords: isothermal nucleic; acid amplification; test; detection ... See more keywords
Diagnostic Yield of Nucleic Acid Amplification Tests in Oral Samples for Pulmonary Tuberculosis: A Systematic Review and Meta-analysis
Sign Up to like & getrecommendations! Published in 2023 at "Open Forum Infectious Diseases"
DOI: 10.1093/ofid/ofad082
Abstract: Abstract The diagnostic accuracy of oral specimen nucleic acid amplification tests (NAATs) for pulmonary tuberculosis (PTB) remains controversial. We performed a systematic review according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, including… read more here.
Keywords: pulmonary tuberculosis; amplification tests; acid amplification; oral samples ... See more keywords
A nucleic acid amplification test‐based strategy does not help inform return to work for healthcare workers with COVID‐19
Sign Up to like & getrecommendations! Published in 2022 at "Influenza and Other Respiratory Viruses"
DOI: 10.1111/irv.13000
Abstract: The objective of this study is to assess the utility of a nucleic acid amplification test‐based approach to shorten isolation of healthcare workers (HCWs) with COVID‐19 in the setting of the highly transmissible omicron variant. read more here.
Keywords: test based; amplification test; acid amplification; nucleic acid ... See more keywords