Deep Learning‐Enhanced Hand‐Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPR
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Science"
DOI: 10.1002/advs.202414918
Abstract: The early detection of high‐risk human papillomavirus (HR‐HPV) is crucial for the assessment and improvement of prognosis in cervical cancer. However, existing PCR‐based screening methods suffer from inadequate accessibility, which dampens the enthusiasm for screening… read more here.
Keywords: detection; hpv; nucleic acid; multiplexed nucleic ... See more keywords
Stretch‐driven microfluidic chip for nucleic acid detection
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27839
Abstract: Molecular diagnosis is an essential means to detect pathogens. The portable nucleic acid detection chip has excellent prospects in places where medical resources are scarce, and it is also of research interest in the field… read more here.
Keywords: microfluidic chip; detection; chip nucleic; acid detection ... See more keywords
Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400572
Abstract: We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5‐fold,… read more here.
Keywords: detection; nucleic acid; sensitivity nucleic; detection via ... See more keywords
Improved Strategies for CRISPR-Cas12-based Nucleic Acids Detection
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Analysis and Testing"
DOI: 10.1007/s41664-022-00212-4
Abstract: The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology. Thus, it is urgent to develop a more simple and efficient nucleic acid detection technology. CRISPR-Cas12 has signal amplification ability, high sensitivity… read more here.
Keywords: based nucleic; detection; cas12 based; nucleic acid ... See more keywords
The point-of-care-testing of nucleic acids by chip, cartridge and paper sensors
Sign Up to like & getrecommendations! Published in 2021 at "Chinese Chemical Letters"
DOI: 10.1016/j.cclet.2021.06.025
Abstract: ABSTRACT Point-of-care nucleic acid testing (POCNAT) has played an important role in the outbreak of infectious diseases (e.g., COVID-19) over recent years. POCNAT aims to realize the rapid, simple and automatic detection of nucleic acid.… read more here.
Keywords: acid detection; nucleic acid; point care; technology ... See more keywords
The why and how of amino acid analytics in cancer diagnostics and therapy.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2016.12.001
Abstract: Pathological alterations in cell functions are frequently accompanied by metabolic reprogramming including modifications in amino acid metabolism. Amino acid detection is thus integral to the diagnosis of many hereditary metabolic diseases. The development of malignant… read more here.
Keywords: cancer; acid detection; amino acid; acid analytics ... See more keywords
An ultrasensitive electrochemiluminescence assay for nucleic acid detection based on carboxyl functionalized polymer dots
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Electroanalytical Chemistry"
DOI: 10.1016/j.jelechem.2021.115743
Abstract: Abstract An electrochemiluminescence (ECL) sensing platform for ultrasensitive detection of miRNA-122 was developed by integrating novel emitters of polymer dots (Pdots) and signal amplification strategies. Firstly, we synthesized a novel polymer with carboxyl group which… read more here.
Keywords: polymer dots; detection; electrochemiluminescence; acid detection ... See more keywords
In situ formation of Ag/Au nanorods as a platform to design a non-aggregation colorimetric assay for uric acid detection in biological fluids
Sign Up to like & getrecommendations! Published in 2020 at "Microchemical Journal"
DOI: 10.1016/j.microc.2020.104642
Abstract: Abstract Herein, we report on a sensitive probe for colorimetric detection of uric acid based on the formation of Ag shell on Au nanorods (Ag/AuNRs) in the presence of uric acid. Uric acid can reduce… read more here.
Keywords: acid detection; uric acid; acid; situ formation ... See more keywords
Specificity Enhancement of Deoxyribonucleic Acid Polymerization for Sensitive Nucleic Acid Detection.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03223
Abstract: Specificity of DNA polymerization plays a critical role in DNA replication and storage of genetic information. Likewise, biotechnological applications, such as nucleic acid detection, DNA amplification, and gene cloning, require high specificity in DNA synthesis… read more here.
Keywords: polymerization; dna; acid detection; nucleic acid ... See more keywords
Automated Microfluidic Nucleic Acid Detection Platform-Integrated RPA-T7-Cas13a for Pathogen Diagnosis.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00242
Abstract: There is a growing urgent need for point-of-care testing (POCT) devices that integrate sample pretreatment and nucleic acid detection in a rapid, economical, and non-labor-intensive way. Here, we have developed an automated, portable nucleic acid… read more here.
Keywords: acid detection; rpa cas13a; detection; nucleic acid ... See more keywords
Rapid Detection of Uropathogens Using an Integrated Multiplex Digital Nucleic Acid Detection Assay Powered by a Digital-to-Droplet Microfluidic Device.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c02578
Abstract: The digital nucleic acid detection assay features the capability of absolute quantitation without the need for calibration, thereby facilitating the rapid identification of pathogens. Although several integrated digital nucleic acid detection techniques have been developed,… read more here.
Keywords: detection; nucleic acid; digital droplet; acid detection ... See more keywords