Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_4
Abstract: Formin is a highly processive motor that offers very unique features to control the elongation of actin filaments. When bound to the filament barbed-end, it enhances the addition of profilin-actin from solution to dramatically accelerate… read more here.
Keywords: actin assembly; actin; study; using microfluidics ... See more keywords
Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.
Sign Up to like & getrecommendations! Published in 2021 at "Biophysical journal"
DOI: 10.1016/j.bpj.2021.06.023
Abstract: Formins generate unbranched actin filaments by a conserved, processive actin assembly mechanism. Most organisms express multiple formin isoforms that mediate distinct cellular processes and facilitate actin filament polymerization by significantly different rates, but how these… read more here.
Keywords: actin; actin assembly; fission yeast; cytokinesis ... See more keywords
Actin Assembly around the Shigella-Containing Vacuole Promotes Successful Infection
Sign Up to like & getrecommendations! Published in 2020 at "Cell Reports"
DOI: 10.1016/j.celrep.2020.107638
Abstract: Summary The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around… read more here.
Keywords: actin assembly; containing vacuole; actin; infection ... See more keywords
Nuclear F-actin assembly on damaged chromatin is regulated by DYRK1A and Spir1 phosphorylation
Sign Up to like & getrecommendations! Published in 2024 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkae574
Abstract: Abstract Nuclear actin-based movements support DNA double-strand break (DSB) repair. However, molecular determinants that promote filamentous actin (F-actin) formation on the damaged chromatin remain undefined. Here we describe the DYRK1A kinase as a nuclear activity… read more here.
Keywords: actin assembly; dsb; nuclear actin; actin ... See more keywords
Functional Mimicry of Eukaryotic Actin Assembly by Pathogen Effector Proteins
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms231911606
Abstract: The actin cytoskeleton lies at the heart of many essential cellular processes. There are hundreds of proteins that cells use to control the size and shape of actin cytoskeletal networks. As such, various pathogens utilize… read more here.
Keywords: actin; actin assembly; assembly pathogen; eukaryotic actin ... See more keywords
Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.49774
Abstract: The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) that are stockpiled for embryonic development. We showed that breakdown of this specialized… read more here.
Keywords: starfish oocytes; assembly ruptures; actin assembly; nuclear membranes ... See more keywords