Force and Power of a Synthetic Myosin II-Based Machine
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.656
Abstract: The function as a collective motor of skeletal muscle myosin II is studied in vitro with a synthetic bio-machine, consisting of an ensemble of myosin motors interacting with a single actin filament attached with the… read more here.
Keywords: machine; myosin; myosin motors; power ... See more keywords
Real-Time Single-Molecule Kinetic Analyses of AIP1-Enhanced Actin Filament Severing in the Presence of Cofilin.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2018.11.010
Abstract: Rearrangement of actin filaments by polymerization, depolymerization, and severing is important for cell locomotion, membrane trafficking, and many other cellular functions. Cofilin and actin-interacting protein 1 (AIP1; also known as WDR1) are evolutionally conserved proteins… read more here.
Keywords: molecule; filament severing; actin filament; cofilin ... See more keywords
Actin Nanobodies Uncover the Mystery of Actin Filament Dynamics in Toxoplasma gondii.
Sign Up to like & getrecommendations! Published in 2017 at "Trends in parasitology"
DOI: 10.1016/j.pt.2017.06.007
Abstract: While the intracellular parasite Toxoplasma relies on a divergent actomyosin motor to support unique speeds in directional movement, the dynamics and architecture of parasite actin filaments remain a much-discussed issue. Using actin chromobodies, Periz et… read more here.
Keywords: uncover mystery; nanobodies uncover; actin nanobodies; filament dynamics ... See more keywords
mDia1 senses both force and torque during F-actin filament polymerization
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01745-4
Abstract: Formins, an important family of force-bearing actin-polymerizing factors, function as homodimers that bind with the barbed end of actin filaments through a ring-like structure assembled from dimerized FH2 domains. It has been hypothesized that force… read more here.
Keywords: actin; actin polymerization; force; actin filament ... See more keywords
Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-13268-1
Abstract: Cellular actin networks can be rapidly disassembled and remodeled in a few seconds, yet in vitro actin filaments depolymerize slowly over minutes. The cellular mechanisms enabling actin to depolymerize this fast have so far remained… read more here.
Keywords: associated protein; depolymerization; cyclase associated; cofilin ... See more keywords
4polar-STORM polarized super-resolution imaging of actin filament organization in cells
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-27966-w
Abstract: Single-molecule localization microscopy provides insights into the nanometer-scale spatial organization of proteins in cells, however it does not provide information on their conformation and orientation, which are key functional signatures. Detecting single molecules’ orientation in… read more here.
Keywords: 4polar storm; microscopy; actin filament; storm ... See more keywords
Celebrating 20 years of live single-actin-filament studies with five golden rules
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2109506119
Abstract: The precise assembly and disassembly of actin filaments is required for several cellular processes, and their regulation has been scrutinized for decades. Twenty years ago, a handful of studies marked the advent of a new… read more here.
Keywords: single actin; actin; celebrating years; years live ... See more keywords
Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2202723119
Abstract: Significance Actin filament nucleation by Arp2/3 complex must be triggered by activators like WASP family proteins. Understanding how WASP proteins activate Arp2/3 complex has been a major challenge due to a lack of high-resolution structures… read more here.
Keywords: structure; microscopy; actin filament; arp2 complex ... See more keywords
Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2206722119
Abstract: Significance Arp2/3 complex is a protein assembly with seven subunits that forms actin filament branches on the sides of preexisting “mother” actin filaments. This process generates growing actin filaments for cellular locomotion and intracellular movements.… read more here.
Keywords: resolution cryo; branch; actin filament; arp2 complex ... See more keywords
Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1101/2022.06.28.497909
Abstract: The fine regulation of actin polymerization is essential to control cell motility, architecture and to perform essential cellular functions. Formins are key regulators of actin filament assembly, known to processively elongate filament barbed ends and… read more here.
Keywords: fh2; filament barbed; barbed ends; actin filament ... See more keywords
Variation of actin filament length in dogs
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Anatomy"
DOI: 10.1111/joa.12961
Abstract: As the smallest functional unit of force production, the sarcomeres are important in determining muscle function. Actin filament lengths, which are important in determining optimal sarcomere length for a species, have not yet been reported… read more here.
Keywords: length dogs; length; variation; muscle ... See more keywords