Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells.
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DOI: 10.1007/978-1-0716-1258-3_22
Abstract: Bioluminescence resonance energy transfer (BRET) is a commonly used assay system for studying protein-protein interactions. The present protocol introduces a conceptually unique ligand-activatable BRET system (termed BRET9), where a full-length artificial luciferase variant 23 (ALuc23),… read more here.
Keywords: protein; system; activatable bret9; mammalian cells ... See more keywords