Fluorescently labeled prey surrogates in combination with fluorescence‐activated cell sorting successfully discriminate actively feeding mixotrophs in a lake water sample
Sign Up to like & getrecommendations! Published in 2024 at "Limnology and Oceanography"
DOI: 10.1002/lno.12545
Abstract: Mixotrophic protists are capable of acting both as primary producers and primary consumers at the base of the aquatic food web, thus constituting key organisms in ecosystems where they are abundant. However, their identity, abundance,… read more here.
Keywords: water; activated cell; cell sorting; labeled prey ... See more keywords
HIF‐1α mediates CXCR4 transcription to activate the AKT/mTOR signaling pathway and augment the viability and migration of activated B cell‐like diffuse large B‐cell lymphoma cells
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Carcinogenesis"
DOI: 10.1002/mc.23515
Abstract: Diffuse large B‐cell lymphoma (DLBCL) is the most common lymphoid malignancy with a high relapse rate. We previously found that C‐X‐C motif chemokine receptor 4 (CXCR4) was highly expressed in DLBCL and associated with poor… read more here.
Keywords: viability migration; cell; activated cell; cell like ... See more keywords
Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2217-9_3
Abstract: Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this… read more here.
Keywords: activated cell; fluorescence activated; cell sorting; cell ... See more keywords
Fluorescence-Activated Cell Sorting of Murine Mammary Cancer Stem-Like Cell Subpopulations with HIF Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7665-2_22
Abstract: Fluorescence-activated cell sorting (FACS) is a common method to identify and to isolate subpopulations within a complex mixture of cells based on their light scatter and fluorescent staining profiles. FACS is widely used to enrich… read more here.
Keywords: activated cell; cell sorting; fluorescence activated; cancer ... See more keywords
Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7714-7_9
Abstract: Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the… read more here.
Keywords: cardiac progenitors; activated cell; isolating embryonic; cell sorting ... See more keywords
Identifying Differentially Expressed Genes Using Fluorescence-Activated Cell Sorting (FACS) and RNA Sequencing from Low Input Samples.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8618-7_6
Abstract: Cell type-specific gene expression profiles are useful for understanding genes that are important for the development of different tissues and organs. Here, we describe how to perform fluorescence-activated cell sorting (FACS) on Arabidopsis root protoplasts… read more here.
Keywords: activated cell; cell sorting; sorting facs; rna sequencing ... See more keywords
Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_14
Abstract: In the past decades, monoclonal antibodies have made an unprecedented transformation from research tools to a rapidly growing class of therapeutics. Advancements in the yeast surface display platform enable the selection of favorable mouse or… read more here.
Keywords: activated cell; antibody; selection; cell receptor ... See more keywords
Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_15
Abstract: Anti-hapten antibodies are widely used as specific immunochemical detection tools in a variety of clinical and environmental analyses. The sensitivity, however, is limited due to the resulting antibody affinities to the haptens which, in turn,… read more here.
Keywords: activated cell; hapten antibodies; cell sorting; fluorescence activated ... See more keywords
Development overview of Raman-activated cell sorting devoted to bacterial detection at single-cell level.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-11081-1
Abstract: Understanding the metabolic interactions between bacteria in natural habitat at the single-cell level and the contribution of individual cell to their functions is essential for exploring the dark matter of uncultured bacteria. The combination of… read more here.
Keywords: single cell; activated cell; raman activated; cell sorting ... See more keywords
Intelligent Image-Activated Cell Sorting
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.08.028
Abstract: A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We… read more here.
Keywords: activated cell; cell sorting; cell; image activated ... See more keywords
On-Chip Photoacoustics-Activated Cell Sorting (PA-ACS) for Label-Free and High-Throughput Detection and Screening of Microalgal Cells.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c04665
Abstract: Microalgae play a crucial role in global carbon cycling as they convert carbon dioxide into various valuable macromolecules. Among them, Haematococcus pluvialis (H. pluvialis) is the richest natural source of astaxanthin (AXT), which is a… read more here.
Keywords: photoacoustics activated; high throughput; activated cell; cell ... See more keywords