UV-Vis Spectra-Activated Droplet Sorting for Label-Free Chemical Identification and Collection of Droplets.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c02822
Abstract: We introduce the UV-vis spectra-activated droplet sorter (UVADS) for high-throughput label-free chemical identification and enzyme screening. In contrast to previous absorbance-based droplet sorters that relied on single-wavelength absorbance in the visible range, our platform collects… read more here.
Keywords: spectra activated; droplet; vis spectra; chemical ... See more keywords
Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00103
Abstract: Synthetic biology aims to improve human health and the environment by repurposing biological enzymes for use in practical applications. However, natural enzymes often function with suboptimal activity when engineered into biological pathways or challenged to… read more here.
Keywords: droplet sorting; biology; activated droplet; directed evolution ... See more keywords
Positive dielectrophoresis–based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo
Sign Up to like & getrecommendations! Published in 2020 at "Science Advances"
DOI: 10.1126/sciadv.abb3521
Abstract: We introduce a novel, high-speed Raman-activated cell sorter (pDEP-RADS) that screens enzymes in vivo. The potential of Raman-activated cell sorting (RACS) is inherently limited by conflicting demands for signal quality and sorting throughput. Here, we… read more here.
Keywords: raman; positive dielectrophoresis; raman activated; activated droplet ... See more keywords