Immobilization of Ulp1 protease on NHS-activated Sepharose: a useful tool for cleavage of the SUMO tag of recombinant proteins
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DOI: 10.1007/s10529-017-2330-5
Abstract: ObjectiveTo fabricate an active and stable enzyme through covalent immobilization, a Ubl-specific protease (Ulp1) was used to cleave small ubiquitin-like modifier (SUMO) fusion proteins.ResultsWe immobilized Ulp1 on N-hydroxysuccinimide (NHS)-activated Sepharose with a coupling efficiency of… read more here.
Keywords: useful tool; activated sepharose; cleavage; nhs activated ... See more keywords