Replacement of Loops at the Entrance of the Active Pocket of Streptococcus thermophilus 4,6-α-Glucanotransferase Changes Its Catalytic Activity and Product Specificity.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c00803
Abstract: To explore the roles of loops around active pocket in the reuteran type 4,6-α-glucanotransferase (StGtfB) from S. thermophilus, they were individually or simultaneously replaced with those of an isomalto/maltopolysaccharides type 4,6-α-glucanotransferase from L. reuteri. StGtfB… read more here.
Keywords: active pocket; glucanotransferase; activity product; replacement ... See more keywords
Residue-Orientation-Dependent Dynamics and Selectivity of Active Pocket in Microbe Class I Terpene Cyclases
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DOI: 10.1021/acs.jcim.0c00159
Abstract: Microbe class I terpene cyclases (TPCs) are responsible for deriving numerous functionally and structurally diverse groups of terpenoids natural products. The conformational change of their active pockets from "open" state to "closed" state upon substrate… read more here.
Keywords: residue orientation; pocket; microbe class; active pocket ... See more keywords
Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency
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DOI: 10.1128/aem.00220-23
Abstract: In this study, the active pocket was largely remodeled based on the ALF-scanning strategy we developed. It is believed that ALF-scanning not only could be employed for substrate preference modification but might also play a… read more here.
Keywords: aromatic nitrile; alf scanning; active pocket; preference ... See more keywords