Analysis of 3'-Phosphoadenosine 5'-Phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1398-6_51
Abstract: 3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs) play an important role in transporting 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the universal sulfuryl donor for sulfation, from the cytosol into the lumen of the Golgi apparatus. Here, we describe three methods for… read more here.
Keywords: real time; phosphosulfate; activity assay; phosphoadenosine phosphosulfate ... See more keywords
Automated chromatographic laccase-mediator-system activity assay
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0423-6
Abstract: AbstractTo study the interaction of laccases, mediators, and substrates in laccase-mediator systems (LMS), an on-line measurement was developed using high performance anion exchange chromatography equipped with a CarboPac™ PA 100 column coupled to pulsed amperometric… read more here.
Keywords: mediator; laccase; activity assay; automated chromatographic ... See more keywords
Development of a colorimetric PNGase activity assay.
Sign Up to like & getrecommendations! Published in 2019 at "Carbohydrate research"
DOI: 10.1016/j.carres.2018.11.007
Abstract: PNGases are crucial targets and valuable tools in analyzing asparagine-linked carbohydrate moieties (N-glycans) of glycoproteins. Activity tests of PNGases have been little improved since their discovery four decades ago, and still rely on observing deglycosylation… read more here.
Keywords: colorimetric pngase; activity assay; development colorimetric; pngase ... See more keywords
Increasing the sensitivity of the human microvesicle tissue factor activity assay.
Sign Up to like & getrecommendations! Published in 2019 at "Thrombosis research"
DOI: 10.1016/j.thromres.2019.07.011
Abstract: INTRODUCTION The TF-FVIIa complex is the primary activator of coagulation. Elevated levels of microvesicle (MV) bearing tissue factor (TF)-dependent procoagulant activity are detectable in patients with an increased risk of thrombosis. Several methods have been… read more here.
Keywords: microvesicle; activity; tissue factor; sensitivity ... See more keywords
Rational Design of Dual-Emission Lanthanide Metal-Organic Framework for Visual Alkaline Phosphatase Activity Assay.
Sign Up to like & getrecommendations! Published in 2021 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.1c00134
Abstract: The alkaline phosphatase (ALP) activity assay is very significant for disease diagnosis and biomedical research. Lanthanide metal-organic framework (Ln-MOF) based fluorescence sensors have great application potential in ALP activity assays. However, it is critical but… read more here.
Keywords: activity assay; rational design; activity; dual emission ... See more keywords
Investigation of biological activities of the flowers of Lagerstroemia speciosa, the Jarul flower of Bangladesh
Sign Up to like & getrecommendations! Published in 2018 at "BMC Complementary and Alternative Medicine"
DOI: 10.1186/s12906-018-2286-6
Abstract: BackgroundLagerstroemia speciosa (L.) Pers. (Family: Lythraceae) is used in traditional medicine in the treatment of diarrhea, diabetes and other diseases. The study was performed to conduct antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial, peripheral and central… read more here.
Keywords: medicine; biological activities; soluble fraction; activity assay ... See more keywords
A direct fluorometric activity assay for lipid kinases and phosphatases.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of lipid research"
DOI: 10.1194/jlr.d120000794
Abstract: Lipid kinases and phosphatases play key roles in cell signaling and regulation, are implicated in many human diseases, and are thus attractive targets for drug development. Currently, no direct in vitro activity assay is available… read more here.
Keywords: kinases phosphatases; direct fluorometric; fluorometric activity; activity assay ... See more keywords
Antimicrobial Activity Assay of Liposomal Lipopolysaccharide Extracted from Escherichia coli.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of Razi Institute"
DOI: 10.22092/ari.2022.358103.2152
Abstract: Escherichia coli (E. coli) is a bacterial bacillus known to be negative for the gram dye, and it is part of the normal flora inside the human body. The current study aimed to investigate the possibility… read more here.
Keywords: activity assay; antimicrobial activity; escherichia coli; liposomal lipopolysaccharide ... See more keywords
A label-free LC/MS-based enzymatic activity assay for the detection of PDE5A inhibitors
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2023.1097027
Abstract: Phosphodiesterase type 5 (PDE5), a cyclic nucleotide phosphodiesterase, controls the duration of the cyclic guanosine monophosphate (cGMP) signal by hydrolyzing cGMP to GMP. Inhibiting the activity of PDE5A has proven to be an effective strategy… read more here.
Keywords: activity assay; pde5a inhibitors; activity; based enzymatic ... See more keywords
A fast, sensitive and fluorescent LPMO activity assay
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1128470
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are industrially relevant enzymes that utilize a copper co-factor and an oxygen species to break down recalcitrant polysaccharides. These enzymes are secreted by microorganisms and are used in lignocellulosic refineries. As… read more here.
Keywords: activity assay; sensitive fluorescent; fast sensitive; activity ... See more keywords
Validation of Aspartylglucosaminidase Activity Assay for Human Serum Samples: Establishment of a Biomarker for Diagnostics and Clinical Studies
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24065722
Abstract: Novel treatment strategies are emerging for rare, genetic diseases, resulting in clinical trials that require adequate biomarkers for the assessment of the treatment effect. For enzyme defects, biomarkers that can be assessed from patient serum,… read more here.
Keywords: activity assay; serum; serum samples; activity ... See more keywords