QM/MM Simulations Reveal the Determinants of Carbapenemase Activity in Class A β-Lactamases
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DOI: 10.1021/acsinfecdis.2c00152
Abstract: β-lactam antibiotic resistance in Gram-negative bacteria, primarily caused by β-lactamase enzymes that hydrolyze the β-lactam ring, has become a serious clinical problem. Carbapenems were formerly considered “last resort” antibiotics because they escaped breakdown by most… read more here.
Keywords: carbapenemase activity; class; enzyme; mechanics ... See more keywords
Structural basis for substrate specificity and carbapenemase activity of OXA-48 Class D β-lactamase.
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DOI: 10.1021/acsinfecdis.9b00304
Abstract: Carbapenem-hydrolyzing class D β-lactamases (CHDLs) are a diverse family of enzymes that are rapidly becoming the predominant cause of bacterial resistance against β-lactam antibiotics in many regions of the world. OXA-48, an atypical member of… read more here.
Keywords: carbapenemase activity; class; acyl enzyme; product ... See more keywords
Tautomer-Specific Deacylation and Ω-Loop Flexibility Explain the Carbapenem-Hydrolyzing Broad-Spectrum Activity of the KPC-2 β-Lactamase
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1101/2022.11.11.516172
Abstract: KPC-2 (Klebsiella pneumoniae carbapenemase-2) is a globally disseminated serine-β-lactamase (SBL) responsible for extensive β-lactam antibiotic resistance in Gram-negative pathogens. SBLs inactivate β-lactams via a mechanism involving a hydrolytically labile covalent acyl-enzyme intermediate. Carbapenems, the most… read more here.
Keywords: tautomer; carbapenem; deacylation; activity ... See more keywords