Optimisation of a high-throughput fluorescamine assay for detection of N-acyl-l-homoserine lactone acylase activity.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.10.029
Abstract: N-acyl-l-homoserine lactone (AHL) acylases are a well-known group of enzymes that disrupt quorum sensing in Gram-negative bacteria by degrading AHL signalling molecules. This degradation of signalling molecules (termed 'quorum quenching') has potential uses in the… read more here.
Keywords: assay detection; acyl homoserine; homoserine lactone; acylase activity ... See more keywords
Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.9b00315
Abstract: Sirtuins (SIRTs) are a family of nicotinamide adenine dinucleotide-dependent histone deacetylases that serve as epigenetic regulators of many physiological processes. Recent studies have shown that in addition to their well-known deacetylase activity, sirtuins also exhibit… read more here.
Keywords: fluorescence probe; probe sfp3; defatty acylase; acylase activity ... See more keywords