Nanodisc scaffold peptide (NSPr) replaces detergent by reconstituting acyl-CoA:cholesterol acyltransferase 1 into peptidiscs.
Sign Up to like & getrecommendations! Published in 2020 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2020.108518
Abstract: To conduct biochemical studies in vitro, membrane proteins (MPs) must be solubilized with detergents. While detergents are great tools, they can also inhibit the biological activity and/or perturb oligomerization of individual MPs. Nanodisc scaffold peptide… read more here.
Keywords: acat1 peptidiscs; cholesterol; detergent; peptidiscs ... See more keywords
Structure, mechanism, and inhibition of Hedgehog acyltransferase
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Cell"
DOI: 10.1016/j.molcel.2021.11.018
Abstract: Summary The Sonic Hedgehog (SHH) morphogen pathway is fundamental for embryonic development and stem cell maintenance and is implicated in various cancers. A key step in signaling is transfer of a palmitate group to the… read more here.
Keywords: structure mechanism; hedgehog acyltransferase; structure; mechanism inhibition ... See more keywords
A novel dual-functional enzyme Lip10 catalyzes lipase and acyltransferase activities in the oleaginous fungus Mucor circinelloides.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.9b05617
Abstract: Lipases or triacylglycerol lipases belong to the α/β-hydrolases superfamily, which are enzymes capable of catalyzing the hydrolysis of the ester bond between fatty acids and glycerol. Interestingly, some lipases have been found to not only… read more here.
Keywords: mucor circinelloides; dual functional; acyltransferase; activity ... See more keywords
Targeting Sterol O-Acyltransferase/Acyl-CoA:Cholesterol Acyltransferase (ACAT): A Perspective on Small-Molecule Inhibitors and Their Therapeutic Potential.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.2c01265
Abstract: Sterol O-acyltransferase (SOAT) is a membrane-bound enzyme that aids the esterification of cholesterol and fatty acids to cholesterol esters. SOAT has been studied extensively as a potential drug target, since its inhibition can serve as… read more here.
Keywords: sterol acyltransferase; targeting sterol; acyltransferase acyl; acyl coa ... See more keywords
Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis
Sign Up to like & getrecommendations! Published in 2023 at "Plant Physiology"
DOI: 10.1093/plphys/kiad091
Abstract: Abstract Glycerolipids are the most abundant lipids in microalgae, and glycerol-3-phosphate:acyl-CoA acyltransferase (GPAT) plays an important role in their biosynthesis. However, the biochemical and biological functions of algal GPAT remain poorly characterized. Here, we characterized… read more here.
Keywords: biosynthesis; high value; crgpater; role ... See more keywords
Diacylglycerol Acyltransferase 1 Is Regulated by Its N-Terminal Domain in Response to Allosteric Effectors1[OPEN]
Sign Up to like & getrecommendations! Published in 2017 at "Plant Physiology"
DOI: 10.1104/pp.17.00934
Abstract: The N-terminal domain of Brassica diacylglycerol acyltransferase1 binds CoA and acyl-CoA, contributing to feedback inhibition and activation, respectively, of triacylglycerol biosynthesis. Diacylglycerol acyltransferase 1 (DGAT1) is an integral membrane enzyme catalyzing the final and committed… read more here.
Keywords: terminal domain; diacylglycerol; acyl coa; coa ... See more keywords
Allosteric modulation of the substrate specificity of acyl-CoA wax alcohol acyltransferase 2
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Lipid Research"
DOI: 10.1194/jlr.m073692
Abstract: The esterification of alcohols with fatty acids is a universal mechanism to form inert storage forms of sterols, di- and triacylglycerols, and retinoids. In ocular tissues, formation of retinyl esters is an essential step in… read more here.
Keywords: acyl coa; specificity; wax alcohol; coa wax ... See more keywords
Structural Insight of KSIII (β-Ketoacyl-ACP Synthase)-like Acyltransferase ChlB3 in the Biosynthesis of Chlorothricin
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27196405
Abstract: Chlorothricin (CHL) belongs to a spirotetronate antibiotic family produced by Streptomyces antibioticus that inhibits pyruvate carboxylase and malate dehydrogenase. For the biosynthesis of CHL, ChlB3 plays a crucial role by introducing the 6-methylsalicylic acid (6MSA)… read more here.
Keywords: ksiii ketoacyl; biosynthesis; insight ksiii; structural insight ... See more keywords