Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology Journal"
DOI: 10.1002/biot.202200533
Abstract: CRISPR/Cas9 system has been applied as an effective genome‐targeting technology. By fusing deaminases with Cas9 nickase (nCas9), various cytosine and adenine base editors (CBEs and ABEs) have been successfully developed that can efficiently induce nucleotide… read more here.
Keywords: cytosine adenine; pam sequences; base editors; adenine base ... See more keywords
High-Efficiency and Multiplex Adenine Base Editing in Plants Using New TadA Variants.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular plant"
DOI: 10.1016/j.molp.2021.02.007
Abstract: Recently reported adenine base editors (ABEs) exhibit powerful potentials in targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function study and crop breeding. However, editing efficiency varies significantly… read more here.
Keywords: tada variants; base; adenine base; high efficiency ... See more keywords
Adenine Base Editing System for Pseudomonas and Prediction Workflow for Protein Dysfunction via ABE.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00066
Abstract: Pseudomonas is a large genus that inhabits diverse environments due to its distinct metabolic versatility. Its applications range from environmental to industrial biotechnology. Molecular tools that allow precise and efficient genetic manipulation are required to… read more here.
Keywords: system; adenine base; dysfunction; prediction ... See more keywords
Targeted exon skipping with AAV-mediated split adenine base editors
Sign Up to like & getrecommendations! Published in 2019 at "Cell Discovery"
DOI: 10.1038/s41421-019-0109-7
Abstract: Techniques for exclusion of exons from mature transcripts have been applied as gene therapies for treating many different diseases. Since exon skipping has been traditionally accomplished using technologies that have a transient effect, it is… read more here.
Keywords: targeted exon; base editors; adenine base; base ... See more keywords
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15892-8
Abstract: CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain… read more here.
Keywords: guide rna; editor; base; base editor ... See more keywords
Precision genome engineering through adenine base editing in plants
Sign Up to like & getrecommendations! Published in 2018 at "Nature Plants"
DOI: 10.1038/s41477-018-0178-x
Abstract: The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis… read more here.
Keywords: base; adenine base; plants precision; genome engineering ... See more keywords
Directed evolution of adenine base editors with increased activity and therapeutic application
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0491-6
Abstract: The foundational adenine base editors (for example, ABE7.10) enable programmable A•T to G•C point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a… read more here.
Keywords: base editors; adenine base; directed evolution; evolution adenine ... See more keywords
In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels
Sign Up to like & getrecommendations! Published in 2021 at "Nature Biotechnology"
DOI: 10.1038/s41587-021-00933-4
Abstract: Most known pathogenic point mutations in humans are C•G to T•A substitutions, which can be directly repaired by adenine base editors (ABEs). In this study, we investigated the efficacy and safety of ABEs in the… read more here.
Keywords: base editing; pcsk9 macaques; editing pcsk9; base ... See more keywords
Small-molecule activators specific to adenine base editors through blocking the canonical TGF-β pathway
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac742
Abstract: Abstract Adenine base editors (ABEs) catalyze A-to-G conversions, offering therapeutic options to treat the major class of human pathogenic single nucleotide polymorphisms (SNPs). However, robust and precise editing at diverse genome loci remains challenging. Here,… read more here.
Keywords: small molecule; activators specific; molecule activators; base editors ... See more keywords