Protein Arginine Methyltransferase 1‐Dependent Labeling and Isolation of Histone H4 through N‐Mustard Analogues of S‐Adenosyl‐l‐methionine
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800477
Abstract: Histones, the fundamental building blocks of nucleosomes, undergo post‐translational modifications and play a major role in the regulation of transcriptional processes. Although the significance of these modifications, including methylation, is widely recognized, little is known… read more here.
Keywords: adenosyl methionine; protein arginine; analogues adenosyl; mustard analogues ... See more keywords
Identification of methionine adenosyltransferase with high diastereoselectivity for biocatalytic synthesis of (S)-S-adenosyl-l-methionine and exploring its relationship with fluorinated biosynthetic pathway.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109881
Abstract: Natural fluorinated products are rare and attract great attention. The de novo fluorometabolites biosynthetic pathway in microbes has been studied. It is revealed that the carbon-fluorine (C-F) bond is formed by an exotic enzyme called… read more here.
Keywords: high diastereoselectivity; methionine; adenosyl methionine; biosynthetic pathway ... See more keywords
Accumulation and Pulse Electron Paramagnetic Resonance Spectroscopic Investigation of the 4-Oxidobenzyl Radical Generated in the Radical S-Adenosyl-l-methionine Enzyme HydG.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00619
Abstract: The radical S-adenosyl-l-methionine (SAM) enzyme HydG cleaves tyrosine to generate CO and CN- ligands of the [FeFe] hydrogenase H-cluster, accompanied by the formation of a 4-oxidobenzyl radical (4-OB•), which is the precursor to the HydG… read more here.
Keywords: hydg; oxidobenzyl radical; adenosyl methionine; resonance ... See more keywords
Insertion of 4-Demethylwyosine in tRNAPhe Catalyzed by the Radical S-Adenosyl-l-methionine Enzyme TYW1 Entails Oxidative Cleavage of Pyruvate to Form CO2.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00519
Abstract: The radical S-adenosyl-l-methionine (SAM) enzyme TYW1 catalyzes the condensation of C-2 and C-3 atoms of pyruvate with N-methylguanosine containing tRNAPhe to form 4-demethylwyosine (imG-14) modified tRNAPhe. The fate of C-1 is not known, and either… read more here.
Keywords: oxidative cleavage; enzyme tyw1; adenosyl methionine; tyw1 ... See more keywords
Leveraging Substrate Promiscuity of a Radical S-Adenosyl-L-methionine RiPP Maturase toward Intramolecular Peptide Cross-Linking Applications
Sign Up to like & getrecommendations! Published in 2022 at "ACS Central Science"
DOI: 10.1021/acscentsci.2c00501
Abstract: Radical S-adenosyl-l-methionine (RS) enzymes operate on a variety of substrates and catalyze a wide range of complex radical-mediated transformations. Radical non-α-carbon thioether peptides (ranthipeptides) are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs).… read more here.
Keywords: radical adenosyl; cross; cys asp; adenosyl methionine ... See more keywords
Class I Methyltransferase VioH Catalyzes Unusual S-Adenosyl-l-methionine Cyclization Leading to 4-Methylazetidinecarboxylic Acid Formation during Vioprolide Biosynthesis.
Sign Up to like & getrecommendations! Published in 2019 at "ACS chemical biology"
DOI: 10.1021/acschembio.8b00958
Abstract: S-Adenosyl-l-methionine (SAM)-dependent methyltransferases are intensely studied because they play important roles in the methylation of biomolecules in all domains of life. In this study, we describe that the methyltransferase VioH from Cysotobacter violaceus catalyzes a… read more here.
Keywords: adenosyl methionine; sam; methyltransferase vioh; methylazetidinecarboxylic acid ... See more keywords
Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c12010
Abstract: The biosynthesis of blasticidin S has drawn attention due to the participation of the radical S-adenosyl methionine (SAM) enzyme BlsE. The original assignment of BlsE as a radical-mediated, redox-neutral decarboxylase is unusual because this reaction… read more here.
Keywords: biosynthesis blasticidin; dehydration; enzyme blse; adenosyl methionine ... See more keywords
Reconstitution and Substrate Specificity of the Radical S-Adenosyl-methionine Thiazole C-Methyltransferase in Thiomuracin Biosynthesis.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b00693
Abstract: Thiomuracin is a thiopeptide antibiotic with potent activity toward Gram-positive drug-resistant bacteria. Thiomuracin is biosynthesized from a precursor peptide, TbtA, by a complex array of posttranslational modifications. One of several intriguing transformations is the C-methylation… read more here.
Keywords: methyltransferase; thiomuracin; radical adenosyl; reconstitution ... See more keywords
A radical S-adenosyl-L-methionine enzyme and a methyltransferase catalyze cyclopropane formation in natural product biosynthesis
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-05217-1
Abstract: Cyclopropanation of unactivated olefinic bonds via addition of a reactive one-carbon species is well developed in synthetic chemistry, whereas natural cyclopropane biosynthesis employing this strategy is very limited. Here, we identify a two-component cyclopropanase system,… read more here.
Keywords: biosynthesis; cyclopropane; methyltransferase; adenosyl methionine ... See more keywords
S-Adenosyl-l-Methionine Overcomes uL3-Mediated Drug Resistance in p53 Deleted Colon Cancer Cells
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22010103
Abstract: Purpose: In order to study novel therapeutic approaches taking advantage of natural compounds showing anticancer and anti-proliferative effects, we focused our interest on S-adenosyl-l-methionine, a naturally occurring sulfur-containing nucleoside synthesized from adenosine triphosphate and methionine… read more here.
Keywords: drug resistance; adenosyl methionine; cancer cells; cancer ... See more keywords