Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700361
Abstract: Structural and activity studies have revealed the dynamic and transient actions of carrier protein (CP) activity in primary and secondary metabolic pathways. CP‐mediated interactions play a central role in nonribosomal peptide biosynthesis, as they serve… read more here.
Keywords: protein; protein interactions; aryl acid; protein protein ... See more keywords
Crystal structure of the adenylation domain from an ε-poly-l-lysine synthetase provides molecular mechanism for substrate specificity.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2022.01.053
Abstract: ε-poly-l-lysine (ε-PL) synthetase (Pls) is a membrane protein that possesses both adenylation and thiolation domains, characteristic of non-ribosomal peptide synthetases (NRPSs). Pls catalyzes the polymerization of l-Lys molecules in a highly specific manner within proteinogenic… read more here.
Keywords: crystal structure; adenylation; adenylation domain; mechanism ... See more keywords
Biosynthetic Incorporation of Non-native Aryl Acid Building Blocks into Peptide Products Using Engineered Adenylation Domains.
Sign Up to like & getrecommendations! Published in 2024 at "ACS chemical biology"
DOI: 10.1021/acschembio.4c00663
Abstract: Nonribosomal peptides (NRPs), one of the most widespread secondary metabolites in nature, with therapeutically significant activities, are biosynthesized by modular nonribosomal peptide synthetases (NRPSs). Aryl acids contribute to the structural diversity of NRPs as well… read more here.
Keywords: adenylation; aryl acid; benzoic acid; acid ... See more keywords
Free Piperazic Acid as a Precursor to Nonribosomal Peptides.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03660
Abstract: Piperazic acid (Piz) is a nonproteinogenic amino acid possessing a rare nitrogen-nitrogen bond. However, little is known about how Piz is incorporated into nonribosomal peptides, including whether adenylation domains specific to Piz exist. In this… read more here.
Keywords: free piperazic; piperazic acid; adenylation; nonribosomal peptides ... See more keywords
Antibacterial drugs: Discovering antibiotics through soil metagenomics
Sign Up to like & getrecommendations! Published in 2018 at "Nature Reviews Drug Discovery"
DOI: 10.1038/nrd.2018.36
Abstract: products with antimicrobial properties has been hampered by the fact that many bacteria cannot be cultured in the laboratory, and those that can do not produce all of their natural products in this setting. Hover… read more here.
Keywords: calcium dependent; natural products; adenylation; calcium ... See more keywords
Re-adenylation by TENT5A enhances efficacy of SARS-CoV-2 mRNA vaccines
Sign Up to like & getrecommendations! Published in 2025 at "Nature"
DOI: 10.1038/s41586-025-08842-1
Abstract: Despite the widespread use of mRNA vaccines against COVID-19, little is known about the metabolism of therapeutic RNAs. Here we use nanopore sequencing1, 2–3 to analyse individual therapeutic mRNA molecules, focusing on their poly(A) tails.… read more here.
Keywords: adenylation; mrna 1273; efficacy; tent5a ... See more keywords
Dysregulation of Alternative Poly-adenylation as a Potential Player in Autism Spectrum Disorder
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2017.00279
Abstract: We present here the hypothesis that alternative poly-adenylation (APA) is dysregulated in the brains of individuals affected by Autism Spectrum Disorder (ASD), due to disruptions in the calcium signaling networks. APA, the process of selecting… read more here.
Keywords: poly adenylation; dysregulation; spectrum disorder; alternative poly ... See more keywords