Characterization and Structural Determination of CmnG‐A, the Adenylation Domain That Activates the Nonproteinogenic Amino Acid Capreomycidine in Capreomycin Biosynthesis
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DOI: 10.1002/cbic.202200563
Abstract: Capreomycidine (Cap) is a nonproteinogenic amino acid and building block of nonribosomal peptide (NRP) natural products. We report the formation and activation of Cap in capreomycin biosynthesis. CmnC and CmnD catalyzed hydroxylation and cyclization, respectively,… read more here.
Keywords: nonproteinogenic amino; capreomycin biosynthesis; cap; capreomycidine ... See more keywords
Crystal structure of the adenylation domain from an ε-poly-l-lysine synthetase provides molecular mechanism for substrate specificity.
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DOI: 10.1016/j.bbrc.2022.01.053
Abstract: ε-poly-l-lysine (ε-PL) synthetase (Pls) is a membrane protein that possesses both adenylation and thiolation domains, characteristic of non-ribosomal peptide synthetases (NRPSs). Pls catalyzes the polymerization of l-Lys molecules in a highly specific manner within proteinogenic… read more here.
Keywords: crystal structure; adenylation; adenylation domain; mechanism ... See more keywords
Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase
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DOI: 10.1186/s13036-019-0195-y
Abstract: BackgroundCreating designer molecules using a combination of select domains from polyketide synthases and/or nonribosomal peptide synthetases (NRPS) continues to be a synthetic goal. However, an incomplete understanding of how protein-protein interactions and dynamics affect each… read more here.
Keywords: adenylation domain; methyltransferase; domain bassianolide; domain ... See more keywords