Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/7651_2020_305
Abstract: Cytonemes are specialized signalling filopodia that have a role in development and cellular differentiation. However, they are not well preserved by standard fixation techniques to study protein localization and interactions. A recent methodological advance has… read more here.
Keywords: adherent cells; cultured adherent; preserving cytonemes; fixation ... See more keywords
Bioreactor-Based Adherent Cells Harvesting from Microcarriers with 3D Printed Inertial Microfluidics.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_444
Abstract: Harvesting adherent cells from microcarriers has become one of the major challenges of the downstream bioprocessing at large scale the current method has high maintenance and operation cost, which are the results of frequent clogging,… read more here.
Keywords: adherent cells; harvesting microcarriers; based adherent; cells harvesting ... See more keywords
FISH in Suspension or in Adherent Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1115-9_4
Abstract: Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic… read more here.
Keywords: adherent cells; fish suspension; biology; suspension adherent ... See more keywords
Machine-Learning-Aided Quantification of Area Coverage of Adherent Cells from Phase-Contrast Images
Sign Up to like & getrecommendations! Published in 2022 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927622000794
Abstract: Abstract The advances in machine learning (ML) software availability, efficiency, and friendliness, combined with the increase in the computation power of personal computers, are harnessed to rapidly and (relatively) effortlessly analyze time-lapse image series of… read more here.
Keywords: adherent cells; machine learning; phase contrast; microscopy ... See more keywords
Multifrequency Optomechanical Stiffness Measurement of Single Adherent Cells on a Solid Substrate with High Throughput.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b02356
Abstract: Mechanical properties of a cell reflect its biological and pathological conditions and there have been active research efforts to develop high-throughput platforms to mechanically characterize single cells. Yet, many of these research efforts are focused… read more here.
Keywords: adherent cells; stiffness; single adherent; high throughput ... See more keywords
Inertial picobalance reveals fast mass fluctuations in mammalian cells
Sign Up to like & getrecommendations! Published in 2017 at "Nature"
DOI: 10.1038/nature24288
Abstract: The regulation of size, volume and mass in living cells is physiologically important, and dysregulation of these parameters gives rise to many diseases. Cell mass is largely determined by the amount of water, proteins, lipids,… read more here.
Keywords: adherent cells; mass; mass fluctuations; mammalian cells ... See more keywords
DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-71265-7
Abstract: It is known that cells grown in 3D are more tolerant to drug treatment than those grown in dispersion, but the mechanism for this is still not clear; cells grown in 3D have opportunities to… read more here.
Keywords: adherent cells; cells grown; cell culture; drug ... See more keywords
Influence of neighboring adherent cells on laminar flow induced shear stress in vitro-A systematic study.
Sign Up to like & getrecommendations! Published in 2017 at "Biomicrofluidics"
DOI: 10.1063/1.4979295
Abstract: Cells experience forces if subjected to laminar flow. These forces, mostly of shear force character, are strongly dependent not only on the applied flow field itself but also on hydrodynamic effects originating from neighboring cells.… read more here.
Keywords: adherent cells; laminar flow; stress; influence ... See more keywords
Aerosol–Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction
Sign Up to like & getrecommendations! Published in 2022 at "Nanomaterials"
DOI: 10.3390/nano12081362
Abstract: Nanoparticle toxicity assessments have moved closer to physiological conditions while trying to avoid the use of animal models. An example of new in vitro exposure techniques developed is the exposure of cultured cells at the… read more here.
Keywords: system; adherent cells; semi adherent; lung surfactant ... See more keywords