Investigation of Mitochondrial ADP-Ribosylation Via Immunofluorescence.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_12
Abstract: ADP-ribosylation is a posttranslational protein modification, involved in various cellular processes, ranging from DNA-damage repair to apoptosis. While its function has been studied amply with respect to genotoxic stress-associated nuclear ADP-ribosylation, the functional relevance of… read more here.
Keywords: ribosylation via; mitochondrial adp; adp ribosylation; ribosylation ... See more keywords
Studying Catabolism of Protein ADP-Ribosylation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6993-7_26
Abstract: Protein ADP-ribosylation is a conserved posttranslational modification that regulates many major cellular functions, such as DNA repair, transcription, translation, signal transduction, stress response, cell division, aging, and cell death. Protein ADP-ribosyl transferases catalyze the transfer… read more here.
Keywords: protein; studying catabolism; adp ribosylation; catabolism protein ... See more keywords
Ric-8A-mediated stabilization of the trimeric G protein subunit Gαi is inhibited by pertussis toxin-catalyzed ADP-ribosylation.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.01.036
Abstract: The heterotrimeric G protein subunit Gαi can be activated by G protein-coupled receptors and the cytosolic protein Ric-8A, the latter of which is also known to prevent ubiquitin-dependent degradation of Gαi. Here we show that… read more here.
Keywords: protein; adp ribosylation; adp ribosylated; protein subunit ... See more keywords
Targeting ADP-ribosylation by PARP inhibitors in acute myeloid leukaemia and related disorders.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical pharmacology"
DOI: 10.1016/j.bcp.2019.04.019
Abstract: Acute myeloid leukaemia (AML) is a highly heterogeneous disease characterized by uncontrolled proliferation, block in myeloid differentiation and recurrent genetic abnormalities. In the search of new effective therapies, identification of synthetic lethal partners of AML… read more here.
Keywords: adp ribosylation; targeting adp; myeloid leukaemia; ribosylation parp ... See more keywords
Systems-wide Analysis of Serine ADP-Ribosylation Reveals Widespread Occurrence and Site-Specific Overlap with Phosphorylation.
Sign Up to like & getrecommendations! Published in 2018 at "Cell reports"
DOI: 10.1016/j.celrep.2018.07.083
Abstract: ADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing… read more here.
Keywords: adpr; adp ribosylation; serine; wide analysis ... See more keywords
Development of an ADP-ribosylation assay for residual toxicity in C. difficile binary toxin CDTa using automated capillary western blot.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113125
Abstract: CDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which is commonly associated with the hypervirulent strain present in Clostridium difficile infections. The mutated form of CDTa, 4mCDTa, is… read more here.
Keywords: binary toxin; difficile; adp ribosylation; residual toxicity ... See more keywords
Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls Adipogenesis.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.08.002
Abstract: Although ADP-ribosylation of histones by PARP-1 has been linked to genotoxic stress responses, its role in physiological processes and gene expression has remained elusive. We found that NAD+-dependent ADP-ribosylation of histone H2B-Glu35 by small nucleolar… read more here.
Keywords: adp ribosylation; histone h2b; functional interplay; h2b ... See more keywords
Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Cell"
DOI: 10.1016/j.molcel.2021.04.028
Abstract: Summary ARH3/ADPRHL2 and PARG are the primary enzymes reversing ADP-ribosylation in vertebrates, yet their functions in vivo remain unclear. ARH3 is the only hydrolase able to remove serine-linked mono(ADP-ribose) (MAR) but is much less efficient… read more here.
Keywords: ribosylation provides; poly adp; adp ribosylation; provides insights ... See more keywords
Regulation of Biomolecular Condensates by Poly(ADP-ribose).
Sign Up to like & getrecommendations! Published in 2023 at "Chemical reviews"
DOI: 10.1021/acs.chemrev.2c00851
Abstract: Biomolecular condensates are reversible compartments that form through a process called phase separation. Post-translational modifications like ADP-ribosylation can nucleate the formation of these condensates by accelerating the self-association of proteins. Poly(ADP-ribose) (PAR) chains are remarkably… read more here.
Keywords: adp ribosylation; regulation biomolecular; par; biomolecular condensates ... See more keywords
ADP-Ribosylation, a Multifaceted Posttranslational Modification Involved in the Control of Cell Physiology in Health and Disease.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical reviews"
DOI: 10.1021/acs.chemrev.7b00122
Abstract: Posttranslational modifications (PTMs) regulate protein functions and interactions. ADP-ribosylation is a PTM, in which ADP-ribosyltransferases use nicotinamide adenine dinucleotide (NAD+) to modify target proteins with ADP-ribose. This modification can occur as mono- or poly-ADP-ribosylation. The… read more here.
Keywords: adp ribose; physiology; modification; adp ribosylation ... See more keywords
The dynamic ADP-ribosylome, phosphoproteome, and interactome in LPS-activated macrophages.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00261
Abstract: We have used mass spectrometry (MS) to characterize protein signaling in lipopolysaccharide (LPS)-stimulated macrophages from human blood, human THP1 cells, mouse bone marrow, and mouse Raw264.7 cells. Protein ADP-ribosylation was truncated down to phosphoribose, allowing… read more here.
Keywords: phosphoproteome interactome; adp ribosylation; ribosylome phosphoproteome; interactome ... See more keywords