Multivalent forms of the ribonuclease H1 hybrid binding domain are high‐affinity binders of RNA–DNA hybrids
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14541
Abstract: The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA–DNA hybrids, which are structures present in cellular R‐loops and participate in diverse biological processes. We engineered multivalent HBD… read more here.
Keywords: rna dna; hybrid binding; high affinity; affinity ... See more keywords
Melatonin binds with high affinity and specificity to beta‐amyloid: LC‐MS provides insight into Alzheimer’s disease treatment
Sign Up to like & getrecommendations! Published in 2021 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13279
Abstract: To study the potential relationship between melatonin and beta‐amyloid (Abeta), we established a liquid chromatography–mass spectrometry (LC‐MS) method to quantitatively analyze melatonin, deuterated isotopes (melatonin‐D4), and internal standard 6‐iodo‐2‐(4'‐dimethylamino‐) phenyl‐imidazo(1,2) pyridine (IMPY) under positive (+)… read more here.
Keywords: melatonin; alzheimer disease; beta amyloid; affinity ... See more keywords
A Bionic‐Homodimerization Strategy for Optimizing Modulators of Protein–Protein Interactions: From Statistical Mechanics Theory to Potential Clinical Translation
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202105179
Abstract: Emerging protein–protein interaction (PPI) modulators have brought out exciting ability as therapeutics in human diseases, but its clinical translation has been greatly hampered by the limited affinity. Inspired by the homodimerize structure of antibody, the… read more here.
Keywords: protein protein; clinical translation; affinity; homodimerization ... See more keywords
High‐Affinity Superantigen‐Based Trifunctional Immune Cell Engager Synergizes NK and T Cell Activation for Tumor Suppression
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DOI: 10.1002/advs.202310204
Abstract: The development of immune cell engagers (ICEs) can be limited by logistical and functional restrictions associated with fusion protein designs, thus limiting immune cell recruitment to solid tumors. Herein, a high affinity superantigen‐based multivalent ICE… read more here.
Keywords: superantigen; affinity; cell; tumor ... See more keywords
Voxelotor (GBT440), a first‐in‐class hemoglobin oxygen‐affinity modulator, has promising and reassuring preclinical and clinical data
Sign Up to like & getrecommendations! Published in 2018 at "American Journal of Hematology"
DOI: 10.1002/ajh.25042
Abstract: Voxelotor, a first-in-class drug, is currently being studied for the treatment of sickle cell disease in the United States and Europe that has received Breakthrough Therapy Designation (BTD) by the Food and Drug Administration (FDA)… read more here.
Keywords: hemoglobin; affinity; voxelotor; first class ... See more keywords
Enhancing Tumor Cell Affinity and Inhibiting Growth With Albumin‐Coated Crosslinked Chitosan Nanoparticle Micelles
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Applied Polymer Science"
DOI: 10.1002/app.56751
Abstract: Chitosan (CS)‐based drug delivery systems often face challenges in obtaining clinical approval. Enhancing the binding affinity of CS‐based carriers to target cells, such as tumor cells, is crucial for their translation from bench to bedside.… read more here.
Keywords: nanoparticle micelles; affinity; drug; tumor ... See more keywords
Decorated magnetic nanoparticles with polyvinyl alcohol brushes modified with metal chelate affinity groups for purification of proteins
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2378
Abstract: Refining is a serious challenge in the production of recombinant proteins, and this study proposes a new and easy strategy for the synthesis of magnetic affinity nanoparticles. First, the nanoparticles attached to polyvinyl alcohol brushes… read more here.
Keywords: affinity; microscopy; polyvinyl alcohol; alcohol brushes ... See more keywords
Rapid Buffer and Ligand Screening for Affinity Chromatography by Multiplexed Surface Plasmon Resonance Imaging.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology journal"
DOI: 10.1002/biot.201700154
Abstract: Protein purifications are often based on the principle of affinity chromatography, where the protein of interest selectively binds to an immobilized ligand. The development of affinity purification requires selecting proper wash and elution conditions. In… read more here.
Keywords: affinity; affinity chromatography; ligand; development ... See more keywords
Affinity-triggered assemblies based on a designed peptide-peptide affinity pair.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201800559
Abstract: Affinity-triggered assemblies rely on affinity interactions as the driving force to assemble physically-crosslinked networks. WW domains are small hydrophobic proteins binding to proline-rich peptides that are typically produced in the insoluble form. Previous works attempted… read more here.
Keywords: affinity; triggered assemblies; based designed; assemblies based ... See more keywords
Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000231
Abstract: Generation of high‐affinity monoclonal antibodies by immunization of chickens is a valuable strategy, particularly for obtaining antibodies directed against epitopes that are conserved in mammals. A generic procedure is established for the humanization of chicken‐derived… read more here.
Keywords: affinity; yeast surface; chicken; humanization ... See more keywords
Macrocyclization enhances affinity of chemokine-binding peptoids.
Sign Up to like & getrecommendations! Published in 2019 at "Biopolymers"
DOI: 10.1002/bip.23244
Abstract: Peptoids that bind to protein targets can be selected from one-bead-one-compound libraries. Macrocyclization has been often used to increase conformational rigidity and binding affinity in both peptides and peptoids. Here we describe a combined strategy… read more here.
Keywords: macrocyclization; affinity; macrocyclization enhances; affinity chemokine ... See more keywords