Affinity capillary electrophoresis and fluorescence spectroscopy for studying enantioselective interactions between omeprazole enantiomer and human serum albumin
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DOI: 10.1002/elps.201600375
Abstract: Baseline separation of omeprazole (OME) enantiomers was achieved by affinity capillary electrophoresis (ACE), using human serum albumin (HSA) as the chiral selector. The influence of several experimental variables such as HSA concentration, the type and… read more here.
Keywords: capillary electrophoresis; fluorescence spectroscopy; fluorescence; human serum ... See more keywords
Application of affinity capillary electrophoresis for charge heterogeneity profiling of biopharmaceuticals
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DOI: 10.1002/elps.201900233
Abstract: Charge heterogeneity profiling is important for the quality control (QC) of biopharmaceuticals. Because of the increasing complexity of these therapeutic entities [1], the development of alternative analytical techniques is needed. In this work, flow‐through partial‐filling… read more here.
Keywords: capillary electrophoresis; heterogeneity profiling; charge heterogeneity; electrophoresis ... See more keywords
Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs
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DOI: 10.1002/elps.202100320
Abstract: In this study, two capillary electrophoresis–based ligand binding assays, namely, mobility shift affinity capillary electrophoresis (ms‐ACE) and capillary electrophoresis‐frontal analysis (CE‐FA), were applied to determine binding parameters of human serum albumin toward small drugs under… read more here.
Keywords: affinity capillary; shift affinity; capillary electrophoresis; electrophoresis frontal ... See more keywords
Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review
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DOI: 10.1002/elps.202200191
Abstract: Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic… read more here.
Keywords: affinity capillary; analysis drug; serum proteins; drug ... See more keywords
Boundary values of binding constants determined by affinity capillary electrophoresis.
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DOI: 10.1002/jssc.202100507
Abstract: This study shows that the upper limit of binding (stability) constants determined by mobility shift affinity capillary electrophoresis can be increased from 104 to 106 -109 L/mol if the lowest possible analyte concentration in samples… read more here.
Keywords: mobility; capillary electrophoresis; binding constants; affinity capillary ... See more keywords