Rapid Buffer and Ligand Screening for Affinity Chromatography by Multiplexed Surface Plasmon Resonance Imaging.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology journal"
DOI: 10.1002/biot.201700154
Abstract: Protein purifications are often based on the principle of affinity chromatography, where the protein of interest selectively binds to an immobilized ligand. The development of affinity purification requires selecting proper wash and elution conditions. In… read more here.
Keywords: affinity; affinity chromatography; ligand; development ... See more keywords
Single‐step purification of cyclotides using affinity chromatography
Sign Up to like & getrecommendations! Published in 2017 at "Peptide Science"
DOI: 10.1002/bip.23010
Abstract: Cyclotides are considered promising scaffolds for drug development owing to their inherent host defence activities and highly stable structure, defined by the cyclic cystine knot. These proteins are expressed as complex mixtures in plants. Although… read more here.
Keywords: purification cyclotides; purification; affinity chromatography; cyclotides using ... See more keywords
FcγRIIIA affinity chromatography complements conventional functional characterization of rituximab
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3304
Abstract: Analytical and functional characterization of batches of biologics/biosimilar products are imperative towards qualifying them for pre‐clinical and clinical investigations. Several orthogonal strategies are employed to characterize the functional attributes of these drugs. However, the use… read more here.
Keywords: affinity chromatography; chromatography; riiia affinity; functional characterization ... See more keywords
Exosomes from human placenta purified by affinity chromatography on sepharose bearing immobilized antibodies against CD81 tetraspanin contain many peptides and small proteins
Sign Up to like & getrecommendations! Published in 2018 at "IUBMB Life"
DOI: 10.1002/iub.1928
Abstract: Exosomes are nanovesicles (40–100 nm) containing various RNAs and different proteins. Exosomes are involved in intracellular communication and immune system function. Exosomes from different sources are usually isolated using standard methods—centrifugation and ultracentrifugations. Exosomes isolated… read more here.
Keywords: sepharose bearing; bearing immobilized; affinity chromatography; antibodies cd81 ... See more keywords
Rapid screening and identification of anticoagulation component from carthami flos by two dimensional thrombin affinity chromatography combined with HPLC-MS/MS.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202100092
Abstract: Carthami flos, commonly known as Honghua in China, is the dried floret of safflower and widely acknowledged as a blood stasis promoting herb. The study aimed at investigating the relationship between thrombin and carthami flos… read more here.
Keywords: carthami flos; thrombin; affinity chromatography; thrombin affinity ... See more keywords
Purification of Antibodies Using Affinity Chromatography.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6412-3_15
Abstract: Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilized to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It… read more here.
Keywords: protein; purification antibodies; affinity chromatography; affinity ... See more keywords
Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7652-2_9
Abstract: Extracellular vesicles (EVs) are secreted nanoscale particles that transfer biomolecular cargo between cells in multicellular organisms. EVs play a variety of roles in intercellular communication and are being explored as potential vehicles for delivery of… read more here.
Keywords: protein; affinity; evs; biology ... See more keywords
Dynamic N-glycoproteome analysis of maize seedling leaves during de-etiolation using Concanavalin A lectin affinity chromatography and a nano-LC–MS/MS-based iTRAQ approach
Sign Up to like & getrecommendations! Published in 2017 at "Plant Cell Reports"
DOI: 10.1007/s00299-017-2209-x
Abstract: Key messageThe identification ofN-glycosylated proteins with information about changes in the level ofN-glycosylation during de-etiolation provides a database that will aid further research on plantN-glycosylation and de-etiolation.AbstractN-glycosylation is one of the most prominent and abundant… read more here.
Keywords: etiolation; nano based; glycosylation; lectin affinity ... See more keywords
Chicken lens development: complete signature of expression of galectins during embryogenesis and evidence for their complex formation with α-, β-, δ-, and τ-crystallins, N-CAM, and N-cadherin obtained by affinity chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Cell and Tissue Research"
DOI: 10.1007/s00441-019-03129-0
Abstract: The emerging multifunctionality of galectins by specific protein-glycan/protein interactions explains the interest to determine their expression during embryogenesis. Complete network analysis of all seven chicken galectins (CGs) is presented in the course of differentiation of… read more here.
Keywords: crystallins cam; chicken; expression; affinity chromatography ... See more keywords
Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.113347
Abstract: The widespread use of immobilized metal-affinity chromatography (IMAC) for fast and efficient purification of recombinant proteins has brought potentially toxic transition elements into common laboratory usage. However, there are few studies on the leaching of… read more here.
Keywords: biochemistry; metal affinity; metal; affinity chromatography ... See more keywords
Miniaturized weak affinity chromatography for ligand identification of nanodiscs-embedded G-protein coupled receptors.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.03.062
Abstract: Biophysical techniques that enable the screening and identification of weak affinity fragments against a target protein are at the heart of Fragment Based Drug Design approaches. In the case of membrane proteins, the crucial criteria… read more here.
Keywords: protein; affinity; weak affinity; nanodiscs embedded ... See more keywords