Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_1
Abstract: Studying protein complexes in vitro requires the production of a relatively pure sample that maintains the full complement, native organization, and function of that complex. This can be particularly challenging to achieve for large, multi-component,… read more here.
Keywords: pore complexes; nuclear pore; affinity; cerevisiae nuclear ... See more keywords
Application of the biotin-labeled toxin mutant for affinity isolation of associated proteins in the mammalian cells.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.12.002
Abstract: Cholera toxin (CT), one of the AB5 bacterial toxin families, is produced by Vibrio cholerae, breeches the intestinal epithelial barrier and enters host epithelial cells to cause the massive secretory diarrhea. This study focused on… read more here.
Keywords: retro translocation; biotin; bacterial toxin; toxin ... See more keywords